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首页> 外文期刊>Clinical immunology: The official journal of the Clinical Immunology Society >Lymphocyte proliferation assays may underestimate antigen responsiveness in human immunodeficiency virus infection.
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Lymphocyte proliferation assays may underestimate antigen responsiveness in human immunodeficiency virus infection.

机译：淋巴细胞增殖测定法可能低估了人类免疫缺陷病毒感染中的抗原反应性。

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The objective of this study was to examine the relationships among lymphocyte proliferation, interferon-gamma (IFN-gamma) production, and apoptosis in peripheral blood mononuclear cells (PBMC) of HIV-1-infected patients and controls. PBMC were prepared from 19 HIV-1-infected patients and 16 healthy controls. Using tetanus toxoid (TT) as a recall antigen, we assessed lymphocyte proliferation using [(3)H]thymidine incorporation after 2, 4, 6, and 7 days' culture and IFN-gamma production in 48-h culture supernatants by ELISA. Apoptosis was measured using TdT-mediated dUTP nick-end labeling. Median stimulation indices (SIs) in HIV-1-infected patients were 2.8 and 3.7 as opposed to 24.9 and 25.1 in healthy controls after 6 and 7 days' culture, respectively (P < 0. 001). Among the controls, peak proliferation was seen after 7 days in culture whereas in patients, SIs peaked at 4 days and fell progressively by days 6 and 7. At 2 and 4 days of stimulation with tetanus, patients' T cells showed increased apoptosis (19 and 25%) vs 12 and 15% apoptosis seen in controls' cells, P < 0.05. Interferon-gamma in 48-h supernatants of TT-stimulated PBMC was comparable among patients and controls. Whereas in our system, 6 and 7 day assays of lymphocyte proliferation provide increasing responses to TT among healthy controls, these durations of culture may underestimate antigen responsiveness in HIV-1 infection. Cell death due to apoptosis may account for this phenomenon. Whether shorter term or longer term assays of lymphocyte responsiveness more accurately reflect in vivo immune competence is unknown. Nonetheless, shorter duration assays may provide a more realistic estimate of the frequency of antigen-reactive cells in persons with HIV-1 infection. Copyright 1999 Academic Press.
机译：这项研究的目的是检查感染HIV-1的患者和对照组的淋巴细胞增殖，干扰素-γ（IFN-γ）产生和外周血单核细胞（PBMC）凋亡之间的关系。 PBMC是从19名感染HIV-1的患者和16名健康对照中制备的。使用破伤风类毒素（TT）作为召回抗原，我们在培养2、4、6和7天后使用[（3）H]胸苷掺入评估了淋巴细胞的增殖，并通过ELISA在48小时培养上清液中产生了IFN-γ。使用TdT介导的dUTP缺口末端标记来测量细胞凋亡。培养6天和7天后，感染HIV-1的患者的中位刺激指数（SIs）分别为2.8和3.7，而健康对照组的中位数刺激指数分别为24.9和25.1（P <0. 001）。在对照组中，培养7天后可见峰值增殖，而在患者中，SI在4天达到峰值，并在第6天和第7天逐渐下降。在破伤风刺激的第2天和第4天，患者的T细胞显示出凋亡增加（19分别为25％和12％和15％（P <0.05）。在TT刺激的PBMC的48小时上清液中的γ干扰素在患者和对照中是可比的。尽管在我们的系统中，健康对照组中淋巴细胞增殖的6天和7天检测对TT的反应增加，但这些培养时间可能低估了HIV-1感染中的抗原反应性。由于凋亡引起的细胞死亡可能是造成这种现象的原因。淋巴细胞反应性的短期或长期测定能否更准确地反映体内免疫能力尚不清楚。尽管如此，持续时间较短的测定法可能会对HIV-1感染者的抗原反应性细胞的频率提供更现实的估计。版权所有1999，学术出版社。

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