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首页> 外文期刊>Clinical immunology: The official journal of the Clinical Immunology Society >Interleukin-4 inhibits an early phase in the HIV-1 life cycle in the human colorectal cell line HT-29.
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Interleukin-4 inhibits an early phase in the HIV-1 life cycle in the human colorectal cell line HT-29.

机译：白细胞介素-4抑制人结肠直肠细胞系HT-29的HIV-1生命周期的早期阶段。

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摘要

Intestinal epithelial cells are continuously in contact with the gut-associated lymphoid tissues (GALT). Gastrointestinal infection by viruses, bacteria and parasites or the presence of an inflammatory bowel disease may influence the GALT cytokine network. However, the effect of the different cytokines on susceptibility of human intestinal epithelial cells to HIV-1 infection remains undefined. We demonstrate here that IL-4 inhibits infection with reporter HIV-1 viruses pseudotyped with NDK-Env without affecting integrated proviral DNA. Furthermore, IL-4 also inhibits, in a dose-dependent manner, infection of HT-29 cells with HIV-1-based AMLV, HTLV-I and VSV-G pseudotypes. A fusion assay showed that this event is not affected by IL-4, thus suggesting that a post-fusion step is affected. A reduction in the completion of DNA retrotranscription indicates that IL-4 may affect this step, or any prior event. Altogether our data indicate that IL-4 is negatively affecting an early post-fusion step in the HIV-1 replication cycle in HT-29 cells.
机译：肠上皮细胞与肠相关淋巴样组织（GALT）持续接触。病毒，细菌和寄生虫的胃肠道感染或炎症性肠病的存在可能影响GALT细胞因子网络。但是，不同细胞因子对人肠上皮细胞对HIV-1感染敏感性的影响尚不确定。我们在这里证明IL-4抑制了NDK-Env假型的报告HIV-1病毒的感染，而不会影响整合的原病毒DNA。此外，IL-4还以剂量依赖性方式抑制基于HIV-1的AMLV，HTLV-1和VSV-G假型感染HT-29细胞。融合测定显示该事件不受IL-4影响，因此表明融合后步骤受到影响。 DNA逆转录完成的减少表明IL-4可能影响该步骤或任何先前事件。总体而言，我们的数据表明IL-4对HT-29细胞中HIV-1复制周期的融合后早期步骤产生了负面影响。

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