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首页> 外文期刊>Journal of Parasitology >COCCIDIAN MEROZOITE TRANSCRIPTOME ANALYSIS FROM EIMERIA MAXIMA IN COMPARISON TO EIMERIA TENELLA AND EIMERIA ACERVULINA
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COCCIDIAN MEROZOITE TRANSCRIPTOME ANALYSIS FROM EIMERIA MAXIMA IN COMPARISON TO EIMERIA TENELLA AND EIMERIA ACERVULINA

机译：极大值球虫的球虫裂殖子转录谱分析，与球虫和球虫的比较

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摘要

With the Eimeria spp. populations that infect chickens used as a model for coccidian biology, we aimed to survey the transcriptome of Eimeria maxima and contrast it to the 2 other Eimeria spp. for which transcriptome data are available, i.e. Eimeria tenella and Eimeria acervulina. The asexual intracellular development stage, the merozoite, was specifically examined, and we used expressed sequence tag (EST) analysis to provide experimental evidence of transcription and a framework for understanding the merozoite stage of E. maxima. Of 2,680 individual ESTs obtained, 48.2% shared most significant (E < 10(-5)) homology to sequences from other apicomplexan species, primarily other Eimeria spp. and Toxoplasma gondii, and 47.5% were unique. Annotation of these ESTs enabled categorization to putative biological function and revealed an emphasis on translation, cytoskeleton, metabolism, signaling, transport, and protein folding, as well as the apicomplexan specific surface antigens and micronemes. Comparative analysis of abundantly expressed transcripts from merozoites of the 3 Elimeria spp. revealed a novel transcript common to all 3. Sharing no significant homology to any other sequence in public databases, this transcript was predicted to encode an Eimeria-specific protein (ESP) with 166-178 amino acids and 58.9-65.1% interspecific identity. A predicted signal peptide was identified, consistent with the assumption that ESP is a secreted protein. These annotated ESTs from E. maxima merozoites provide a resource for in intra- and interspecific comparative analyses that will be useful in distinguishing the unique biology of coccidian parasites in relation to the diverse phylum of Apicomplexa.
机译：与Eimeria spp。我们以感染鸡的种群作为球虫生物学模型，我们旨在调查最大艾美球虫的转录组，并将其与另外两个艾美球虫属进行比较。可获得转录组数据的数据，即艾美耳球虫和小叶艾美球虫。专门检查了无性细胞内发育阶段的裂殖子，我们使用表达序列标签（EST）分析来提供转录的实验证据和理解最大大肠杆菌的裂殖子阶段的框架。在获得的2,680个EST中，有48.2％与其他apicomplexan物种（主要是其他艾美球虫属）的序列具有最显着（E <10（-5））同源性。和弓形虫，并且47.5％是唯一的。这些EST的注释使分类归类为假定的生物学功能，并显示出对翻译，细胞骨架，代谢，信号传导，转运和蛋白质折叠以及apicomplexan特异性表面抗原和微neme的重视。对3种Elimeria spp裂殖子中大量表达的转录本的比较分析。揭示了所有人共有的一种新颖的转录本。与公共数据库中的任何其他序列均无显着同源性，该转录本预计编码具有166-178个氨基酸和58.9-65.1％种间同一性的艾美尔菊特异性蛋白（ESP）。确定了预测的信号肽，与ESP是一种分泌蛋白的假设一致。这些来自大肠埃希氏菌裂殖子的带注释的EST为种内和种间比较分析提供了资源，这将有助于区分球虫寄生虫相对于复杂的蚜虫门的独特生物学。

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