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首页> 外文期刊>Journal of Parasitology >DEVELOPMENT OF A DOUBLE ANTIBODY SANDWICH ELISA ASSAY FOR THE DIAGNOSIS OF ANGIOSTRONGYLIASIS
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DEVELOPMENT OF A DOUBLE ANTIBODY SANDWICH ELISA ASSAY FOR THE DIAGNOSIS OF ANGIOSTRONGYLIASIS

机译：双抗体夹心酶联免疫吸附测定法诊断血管炎的方法的建立

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摘要

With the use of 2 monoclonal antibodies (MAbs) against excretory/secretory (ES) antigens of adult Angiostrongyhis cantonensis, a new method was developed for double antibody sandwich ELISA for the detection of circulating antigens (CAg). To evaluate the sensitivity of the new procedure, the CAg in sera of rats (80) and mice (15) infected with A. cantonensis, as well as CAg in sera of clinically confirmed angiostrongyliasis patients (70), were evaluated. Cross-reaction testing was used to determine the specificity of serum from patients infected with Ascaris lumbricoides, Trichinella spiralis, Toxoplasma gondii, Schistosoma japonicum, Paragonimus westermani, Clonorchis sinensis, Echinococcus granulosus, Spiromora, and Taenia solitan, as well as normal healthy people. The results proved that the sensitivity and the specificity of the new method were totally effective for the detection of A. cantonensis CAg. The assay is highly sensitive, specific, and reproducible, with easy handling and excellent cost effectiveness, and thereby provides a new method for the accurate diagnosis of angiostrongyliasis.
机译：通过使用两种针对成年广州小血管藻的排泄/分泌（ES）抗原的单克隆抗体（MAb），开发了一种用于检测循环抗原（CAg）的双抗体夹心ELISA的新方法。为了评估新方法的敏感性，评估了被广州曲霉感染的大鼠（80只）和小鼠（15只）的血清中的CAg，以及临床确诊的血管平滑肌病患者（70）的血清中的CAg。交叉反应测试用于确定被A虫，旋毛虫，弓形虫，弓形虫，日本血吸虫，湿地百日咳，华支睾吸虫，细粒棘球oc，螺旋体和Ta虫感染的患者以及正常健康人的血清特异性。结果表明，新方法的灵敏性和特异性对检测广州曲霉CAg完全有效。该测定法具有高度的敏感性，特异性和可重复性，操作简便且具有出色的成本效益，从而为准确诊断血管平滑肌病提供了一种新方法。

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