...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Parasitology >Genetic characterization of Toxoplasma gondii isolates from pigs in southwestern China.
【24h】

Genetic characterization of Toxoplasma gondii isolates from pigs in southwestern China.

机译：中国西南地区猪弓形虫的遗传特征。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The genetic diversity of Toxoplasma gondii varies in different geographical regions. Isolates of T. gondii in South America, for example, are genetically and biologically divergent from those in North America and Europe, where the population structure is highly clonal and composed mainly of 3 distinct lineages, i.e., Types I, II, and III. However, little is known of the T. gondii genotypes in the People's Republic of China. Toxoplasma gondii infection in pigs causes significant economic loss and presents a risk for human infection. We conducted a survey to determine the genetic diversity of this parasite in slaughtered pigs from Yunnan Province, southwestern China. In total, 412 DNA samples were extracted from hilar lymph nodes and livers of pigs from slaughterhouses in Yunnan Province in southwest China, 56 of which were found to be positive for the T. gondii SAG3 gene. These positive DNA samples were typed at 10 genetic markers, including 9 nuclear loci, i.e., SAG1, SAG2, SAG3, BTUB, GRA6, L358, PK1, c22-8, c29-2, and an apicoplast locus Apico. Of these, 5 isolates were genotyped with complete data for all loci. Only 1 genotype (ToxoDB 9) was identified, previously reported as a widespread lineage from pigs, cats, and human patients in China. The results indicate that this genotype may be the major T. gondii lineage in China and possibly all of eastern Asia. This is the first report of genetic typing of T. gondii isolates from pigs in China's southwestern Yunnan Province, the results of which have implications for the prevention and control of T. gondii infections in humans and other animals.
机译：弓形虫的遗传多样性在不同的地理区域有所不同。 T的隔离株。例如，南美的刚地犬在遗传和生物学上与北美和欧洲的那些在遗传和生物学上有所不同。在北美和欧洲，种群结构高度克隆，主要由3个不同的世系组成，即I，II和III型。然而，对 T知之甚少。中华人民共和国的冈第（ii）基因型。猪的弓形虫感染会造成重大的经济损失，并有人类感染的危险。我们进行了一项调查以确定该寄生虫在中国西南云南省的出栏猪中的遗传多样性。总共从中国西南的云南省屠宰场的猪的肺门淋巴结和肝脏中提取了412个DNA样品，其中56个T阳性。 gondii SAG3基因。这些阳性DNA样品用10个遗传标记进行分型，包括9个核基因座，即SAG1，SAG2，SAG3，BTUB，GRA6，L358，PK1，c22-8，c29-2和apicoplast位点Apico。其中，对5个分离株进行了基因分型，并提供了所有基因座的完整数据。仅鉴定出一种基因型（ToxoDB 9），先前报道其为中国猪，猫和人类患者的广泛谱系。结果表明该基因型可能是主要的T。在中国，甚至在整个东亚都可以看到贡迪人血统。这是 T基因分型的首次报道。中国云南省西南部地区的猪中分离到弓形虫，其结果对 T的预防和控制具有重要意义。人和其他动物的刚地犬感染。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号