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首页> 外文期刊>Journal of Parasitology >First molecular identification of Diphyllobothrium dendriticum plerocercoids from feral rainbow trout ( Oncorhynchus mykiss) in Chile.
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First molecular identification of Diphyllobothrium dendriticum plerocercoids from feral rainbow trout ( Oncorhynchus mykiss) in Chile.

机译：在智利从野生虹鳟鱼（Oncorhynchus mykiss）中首次鉴定了树突棘生物。

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Between April and June 2009, 1,075 feral rainbow trout from 10 different lakes involved with aquaculture activities in Los Lagos Region, Chile, were inspected for Diphyllobothrium species. All viscera and muscles of the fish were examined using stereomicroscopy; pyloric cecae and stomachs infected with plerocercoids were checked by histology and scanning electron microscopy. Plerocercoids of Diphyllobothrium dendriticum were confirmed by PCR and sequencing of COI and 18S rRNA+ITS1+5.8S rRNA+ITS2 genes for the first time in Chile. Overall prevalence of plerocercoids of D. dendriticum was 9.2% (99/1,075) in Los Lagos Region and 17.4% (99/570) for Chiloe Island. Plerocercoids were not detected in the continental lakes of the Los Lagos Region (Chapo, Rupanco, and Llanquihue). Tarahuin Lake exhibited a prevalence of 50.9% (81/159), Cucao Lake 5.1% (4/79), Natri Lake 4.7% (5/107), Huillinco Lake 3.6% (5/138), and San Antonio Lake 66.7% (4/6). Abundance was 1.1 plerocercoid larvae per fish (1,169 larvae/1,075 fish). All the plerocercoids were found encysted in the viscera of the fish. Plerocercoids were 10.93 (7-16) mm long by 0.40.2 (0.2-0.6) mm wide. The scolex was enlarged, with 2 bothria and a frontal pit. The body was covered with short capilliform filitriches, 4-6 mm long. The Chilean COI and 18SrRNA+ITS1+5.8SrRNA+ITS2 gene sequences indicated 96.34-96.52% and 99% similarity with D. dendriticum sequences, respectively. Diphyllobothrium dendriticum is reported for the first time in freshwater ecosystems as far as 43掳S on Chiloe Island. These findings and previous reports of plerocercoids of Diphyllobothrium spp. in farmed rainbow trout at Tarahuin Lake support the putative life cycle of this parasite in lakes of southern Chile where there are aquaculture activities.
机译：在2009年4月至6月之间，检查了智利洛斯拉各斯地区10个不同湖泊中从事水产养殖活动的1,075只野生虹鳟鱼中的双叶菊属物种。使用体视显微镜检查鱼的所有内脏和肌肉。通过组织学和扫描电子显微镜检查幽门螺杆菌和幽门螺杆菌感染的胃。在智利首次通过PCR和COI和18S rRNA + ITS1 + 5.8S rRNA + ITS2基因的测序确认了树突棘皮的类球菌。洛斯拉各斯地区树突状棘皮动物的总患病率为9.2％（99 / 1,075），奇洛埃岛的患病率为17.4％（99/570）。在洛斯拉各斯地区（Chapo，Rupanco和Llanquihue）的大陆湖中未检出类球菌。塔拉胡恩湖的患病率为50.9％（81/159），库考湖为5.1％（4/79），纳特里湖为4.7％（5/107），惠林科湖为3.6％（5/138），圣安东尼奥湖为66.7％ （4/6）。丰富度为每条鱼1.1个拟除虫藻类幼虫（1,169个幼虫/ 1,075条鱼）。所有的拟螺藻类都发现在鱼的内脏中。乳突长10.93（7-16）mm，宽0.40.2（0.2-0.6）mm。斯库克斯扩大了，有2个两个心和一个额凹。尸体被短毛状丝状纤毛覆盖，长4-6毫米。智利COI和18SrRNA + ITS1 + 5.8SrRNA + ITS2基因序列分别与D. dendriticum序列具有96.34-96.52％和99％的相似性。在奇洛伊岛，首次报道淡水生态系统中的树突状双齿藻达43掳S。这些发现和以前报道的Diphyllobothrium spp的拟除虫菊酯。在塔拉胡因湖养殖的虹鳟鱼中，这种寄生虫的假定生命周期支持智利南部有水产养殖活动的湖泊。

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