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首页> 外文期刊>Journal of Parasitology >THE COMPLETE MITOCHONDRIAL GENOME SEQUENCE OF HEPATOZOON CATESBIANAE (APICOMPLEXA: COCCIDIA: ADELEORINA), A BLOOD PARASITE OF THE GREEN FROG, LITHOBATES (FORMERLY RANA) CLAMITANS
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THE COMPLETE MITOCHONDRIAL GENOME SEQUENCE OF HEPATOZOON CATESBIANAE (APICOMPLEXA: COCCIDIA: ADELEORINA), A BLOOD PARASITE OF THE GREEN FROG, LITHOBATES (FORMERLY RANA) CLAMITANS

机译:绿毛虫的血寄生虫,卡特彼勒肝线虫(线虫:球虫:阿德莱里纳)的完整线粒体基因组序列

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摘要

A complete mitochondrial genome for the blood parasite Hepatozoon catesbianae (Alveolata; Apicomplexa; Coccidia; Adeleorina; Hepatozoidae) was obtained through PCR amplification and direct sequencing of resulting PCR products. The mitochondrial genome of H. catesbianae is 6,397 bp in length and contains 3 protein-coding genes (cytochrome c oxidase subunit I [COI]; cytochrome c oxidase subunit III [COIII]; and cytochrome B [CytB]). Sequence similarities to previously published mitochondrial genomes of other apicomplexan parasites permitted annotation of 23 putative rDNA fragments in the mitochondrial genome of H. catesbianae, 14 large subunit rDNA fragments, and 9 small subunit rDNA fragments. Sequences corresponding to rDNA fragments RNA5, RNA8, RNA11, and RNA19 of Plasmodium falciparum were not identified in the mitrochondrial genome sequence of H. catesbianae. Although the presence of 3 protein-coding regions and numerous putative rDNA fragments is a feature typical for apicomplexan mitochondrial genomes, the mitochondrial genome of H. catesbianae possesses a structure and gene organization that is distinct among the Apicomplexa. This is the first complete mitochondrial genome sequence obtained from any apicomplexan parasite in the suborder Adeleorina.
机译:通过PCR扩增和所得PCR产物的直接测序,获得了血寄生虫Catesbianae(Alveolata; Apicomplexa; Coccidia; Adeleorina; Hepatozoidae)的完整线粒体基因组。马萨诸塞州线虫的线粒体基因组长度为6,397 bp,包含3个蛋白质编码基因(细胞色素c氧化酶亚基I [COI];细胞色素c氧化酶亚基III [COIII];细胞色素B [CytB])。与以前发布的其他apicomplexan寄生虫的线粒体基因组的序列相似性,允许注释Caesbianae线粒体基因组中的23个假定rDNA片段,14个大亚基rDNA片段和9个小亚基rDNA片段。在恶性疟原虫的线粒体基因组序列中未鉴定出与恶性疟原虫的rDNA片段RNA5,RNA8,RNA11和RNA19相对应的序列。尽管存在3个蛋白质编码区和许多推定的rDNA片段是apicomplexan线粒体基因组的典型特征,但Catesbianae的线粒体基因组具有在Apicomplexa中不同的结构和基因组织。这是第一个完整的线粒体基因组序列,该序列是从亚目Adeleorina中的任何apicomplexan寄生虫获得的。

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