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首页> 外文会议> >Intraspecific Comparison of Three Complete Mitochondrial Genome Sequences from the Oyster Mushroom, Pleurotus ostreatus
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Intraspecific Comparison of Three Complete Mitochondrial Genome Sequences from the Oyster Mushroom, Pleurotus ostreatus

机译：牡蛎蘑菇平菇三个完整的线粒体基因组序列的种内比较

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Mitochondria, ATP-producing organelles in cells, have their own independent genomes called the mitochondrial (mt) genome. The effects of the mt genome structure on cellular phenotype during the fruiting body formation of basidiomycetes are presumably important; however, these effects have not been elucidated. Intraspecific variation of mt genome structures, which is indispensable information for assessing the effects of mt genome structure, has been assessed mainly by using restriction fragment length polymorphism (RFLP) patterns. Here, we used complete mt genome sequences of Pleurotus ostreatus isolates to examine intraspecific variation among mt genome structure among three genome types, Ⅰ (accession number: AB573642), Ⅱ (accession number; AB573692), and III (NC_009905), and compared this variation with that determined using RFLP patterns. The mt genome of each P. ostreatus type was a circular DNA molecule ranging in size from 71 947 bp (type Ⅰ) to 73 242 bp (type III). Each of the genomes contained 14 common mt genes, one ribosomal small subunit protein 3 gene, one RNA polymerase gene (only in type Ⅲ), two DNA polymerase genes, and two rRNA genes. Twenty-four tRNA genes were determined to form a common set among the three types of mt genomes. The type Ⅰ mt genome had two additional tRNA genes. The gene orders in each mt genome were almost identical, but each had different open reading frames (ORFs) and introns. Comparison of nucleotide sequences showed that there were many substitutions and indels in the intergenic regions. Furthermore, nucleotide sequence differences were observed in gene-coding regions, the majority of which were related to polymorphisms in amino-acid coding sequences. These results indicated both conservation of mt genome primary structure in P. ostreatus species and sequence polymorphisms that corresponded to the mtDNA RFLPs.
机译：线粒体是细胞中产生ATP的细胞器，有自己独立的基因组，称为线粒体（mt）基因组。据推测，在担子菌子实体形成过程中，mt基因组结构对细胞表型的影响很重要。但是，这些影响尚未阐明。主要通过使用限制性片段长度多态性（RFLP）模式评估了mt基因组结构的种内变异，这对于评估mt基因组结构的影响是必不可少的信息。在这里，我们使用平菇侧耳的完整mt基因组序列检查了三种基因组类型（Ⅰ（登录号：AB573642），Ⅱ（登录号； AB573692）和III（NC_009905）之间的mt基因组结构的种内变异，并进行了比较使用RFLP模式确定的变化。每种P. ostreatus类型的mt基因组是一个环状DNA分子，大小从71 947 bp（Ⅰ型）到73 242 bp（III型）。每个基因组包含14个共同的mt基因，一个核糖体小亚基蛋白3基因，一个RNA聚合酶基因（仅在Ⅲ型中），两个DNA聚合酶基因和两个rRNA基因。确定了二十四个tRNA基因以在三种类型的mt基因组之间形成一个共同的集合。 Ⅰ型mt基因组有两个额外的tRNA基因。每个mt基因组中的基因顺序几乎相同，但是每个具有不同的开放阅读框（ORF）和内含子。核苷酸序列的比较表明，在基因间区域中存在许多取代和插入缺失。此外，在基因编码区中观察到核苷酸序列差异，其中大多数与氨基酸编码序列中的多态性有关。这些结果表明在P. ostreatus物种中mt基因组一级结构的保守性和对应于mtDNA RFLP的序列多态性。

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