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首页> 外文期刊>Journal of Parasitology >VALIDATION OF FLOTAC FOR THE DETECTION AND QUANTIFICATION OF TROGLODYTELLA ABRASSARTI AND NEOBALANTIDIUM COLI IN CHIMPANZEES AND PIGS
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VALIDATION OF FLOTAC FOR THE DETECTION AND QUANTIFICATION OF TROGLODYTELLA ABRASSARTI AND NEOBALANTIDIUM COLI IN CHIMPANZEES AND PIGS

机译：絮凝剂在黑猩猩和猪中检测和定量粗麦草和新铌酸杆菌的验证

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We carried out a calibration of FLOTAC for ciliates Troglodytella abrassarti and Neobalantidium coli based on the selection of a most appropriate flotation solutions, and we also tested its accuracy (i.e., number of detected stages out of known added number of stages to fecal samples) and sensitivity for trophozoites of both ciliates in chimpanzee feces and N. coli cysts in pig feces, compared the detection threshold of FLOTAC with MIF-based sedimentation, and, subsequently, tested the losses of ciliate stages during sample preparation. Nine flotation solutions were evaluated, and ZnSO4 solution (specific gravity [s.g.] 1.2) showed to be the most suitable for trophozoite detection, while Sheather's solution (s.g. 1.33) was selected as most suitable for cysts. The FLOTAC sensitivity in detection of both stages varied: for trophozoites, we found all samples were positive when the intensity of infection 10 trophozoites per gram and higher, whereas for cysts the sensitivity was lower. The accuracy of FLOTAC negatively correlated with infection intensity, and the merthiolate-iodine-formaldehyde sedimentation-based quantification had a lower detection threshold. We demonstrated additional losses of stages of T. abrassarti and N. coli due to their retention in the sediment, which is probably a major reason for discrepancies in the numbers of countable ciliates between both methods. In conclusion, the FLOTAC should not be considered as a gold standard for quantification of intestinal ciliates in primates; instead, we recommend the modified MIF method
机译：我们根据最合适的浮选溶液的选择，对纤毛弯曲杆菌和新巴氏杆菌进行了FLOTAC的校准，并且还测试了其准确性（即从粪便样品已知增加的阶段数中检测出的阶段数）和对黑猩猩粪便中纤毛虫的滋养体和猪粪便中的N. coli囊肿的敏感性，比较了FLOTAC的检测阈值与基于MIF的沉降，并随后测试了样品制备过程中纤毛虫阶段的损失。评估了九种浮选溶液，最适合于滋养体检测的是ZnSO4溶液（比重[s.g.] 1.2），而最适合囊肿的则是Sheather's溶液（s.g. 1.33）。 FLOTAC在两个阶段检测中的敏感性各不相同：对于滋养体，当感染强度达到每克10个滋养体或更高时，我们发现所有样品均为阳性，而对于囊肿，敏感性较低。 FLOTAC的准确性与感染强度呈负相关，基于硫醇盐-碘-甲醛沉淀的定量检测阈值较低。由于它们在沉积物中的滞留，我们证明了A.brassarti和N. coli阶段的额外损失，这可能是两种方法之间可计数纤毛数量差异的主要原因。总之，不应将FLOTAC视为定量灵长类动物肠道纤毛的金标准;相反，我们建议使用修改后的MIF方法

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