A Novel, Simplified Technique to Amplify Eimeria (Coccidia: Apicomplexa) DNA from Oocysts

Gerhold R. W.; McDougald L. R.; Beckstead R. B.

首页> 外文期刊>Journal of Parasitology >A Novel, Simplified Technique to Amplify Eimeria (Coccidia: Apicomplexa) DNA from Oocysts

【24h】

A Novel, Simplified Technique to Amplify Eimeria (Coccidia: Apicomplexa) DNA from Oocysts

机译：一种新颖，简化的技术，从卵囊中扩增艾美球虫（球虫：蚜虫）DNA。

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

A new method to amplify coccidia DNA by polymerase chain reaction (PCR) was developed by placing freeze-thawed oocysts in Readyto-Go PCR bead tubes and using a 5-min initial heat denaturation step. Positive PCR reactions were found in 3 of 3 samples containing 20 or 50 oocysts; when = 5 oocysts were used, 1 of 3 samples was positive. This technique shows potential for effectively and efficiently detecting and identifying oocysts from soil, feces, and other matter.

机译：通过将冻融的卵囊放入Readyto-Go PCR珠管中并使用5分钟的初始热变性步骤，开发了一种通过聚合酶链反应（PCR）扩增球菌DNA的新方法。在包含20或50个卵囊的3个样本中，有3个发现了阳性PCR反应。当使用≤5个卵囊时，3个样本中有1个为阳性。该技术显示了有效，高效地从土壤，粪便和其他物质中检测和鉴定卵囊的潜力。

著录项

来源
《Journal of Parasitology》 |2015年第1期|共2页
作者
Gerhold R. W.; McDougald L. R.; Beckstead R. B.;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类寄生动物、寄生虫学;
关键词

相似文献

外文文献
中文文献
专利

1. A Novel, Simplified Technique to Amplify Eimeria (Coccidia: Apicomplexa) DNA from Oocysts [J] . Gerhold R. W., McDougald L. R., Beckstead R. B. Journal of Parasitology . 2015,第1期

机译：一种新颖，简化的技术，从卵囊中扩增艾美球虫（球虫：蚜虫）DNA。
2. Insight into the ultrastructural organisation of sporulated oocysts of Eimeria nieschulzi (Coccidia, Apicomplexa) [J] . SeemannE., KurthT., EntzerothR. Parasitology Research . 2012,第5期

机译：洞悉艾美尔球虫（Eimeria nieschulzi）（Coccidia，Apicomplexa）的卵囊的超微结构组织
3. Reporter gene expression in cell culture stages and oocysts of Eimeria nieschulzi (Coccidia, Apicomplexa) [J] . Michael Kurth, Rolf Entzeroth Parasitology Research . 2009,第2期

机译：尼姆艾美尔球虫（Coccidia，Apicomplexa）细胞培养阶段和卵囊中的报告基因表达
4. THE ACTIVITY OF ALKALI PHOSPHATASE IN THE JEJUNUM OF CHICKENS DURING EXPERIMENTAL COCCIDIOSIS (Apicomplexa, Eimeriidae, Eimeria tenella) [C] . E.I. AHMADOV, SH.A.TOPCHIYEVA Recent researches in energy, environment, entrepreneurship, innovation . 2011

机译：实验性球虫病（蚜虫，Eimeriidae，Eimeria tenella）在雏鸡空肠中碱性磷酸酶的活性
5. Lipid biology of Cryptosporidium parvum and Eimeria nieschulzi (Apicomplexa). [D] . Mitschler, Ralene Renee. 1994

机译：小隐隐孢子虫和聂氏艾美球虫（Apicomplexa）的脂质生物学。
6. Development of Eimeria nieschulzi (Coccidia Apicomplexa) Gamonts and Oocysts in Primary Fetal Rat Cells [O] . Hong Chen, Stefanie Wiedmer, Sacha Hanig, 2013

机译：胚胎鼠细胞中Eimeria nieschulzi（CoccidiaApicomplexa）Gamonts和卵囊的发育
7. Differentiation of Cryptosporidium parvum Isolates by a Simplified Randomly Amplified Polymorphic DNA Technique [O] . Ming Qi Deng, Dean O. Cliver 1999

机译：通过简化随机扩增的多晶型DNA技术分化隐孢子虫分离物的分化

A Novel, Simplified Technique to Amplify Eimeria (Coccidia: Apicomplexa) DNA from Oocysts

摘要

著录项

相似文献

相关主题

期刊订阅