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首页> 外文期刊>Journal of Parasitology >RHINOSPORIDIUM SEEBERI NUCLEAR CYCLE ACTIVITIES USING CONFOCAL MICROSCOPY
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RHINOSPORIDIUM SEEBERI NUCLEAR CYCLE ACTIVITIES USING CONFOCAL MICROSCOPY

机译：共聚焦显微镜观察塞氏鼻孢子虫核周期活动

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摘要

Rhinosporidium seeberi is an uncultivated Ichthyosporean infecting animals, including humans. Recent studies suggested R. seeberi undergoes synchronized nuclear division without cytokinesis. We used confocal microscopy to investigate R. seeberi nuclear division cycles in formalin-fixed tissues stained with DAPI and phalloidin. We report that R. seeberi nuclei in juvenile and intermediary sporangia synchronously divided without cytokinesis. Intermediary sporangia display numerous 3-4 mu m nuclei at different mitotic stages as well as a thick inner layer with strong affinity for phalloidin. Mature sporangia showed numerous 5-12 mu m cell-walled endospores, each containing a 2-4 mu m in diameter nucleus. Phalloidin did not bind to the inner layers of mature sporangia or endospores. The development of a "germinative zone'' in the inner layer of mature sporangia containing hundreds of nuclei was also confirmed. This study establishes that during the R. seeberi life cycle synchronous nuclear divisions without cytokinesis takes place, resulting in the formation of thousands of nuclei. Cytokinesis, on the other hand, is a 1-time event and occurs in the latest stages of intermediate sporangia, after the formation of thousands of nuclei and just before mature sporangia development.
机译：Seeberi Rhinosporidium seeberi是一种未经培养的鱼孢子感染动物，包括人类。最近的研究表明，Seeberi发生核分裂，没有胞质分裂。我们使用共聚焦显微镜研究了用DAPI和鬼笔环肽染色的福尔马林固定组织中的R. seeberi核分裂周期。我们报告R. Seeberi核在少年和中间孢子囊同步分裂而没有胞质分裂。中间孢子囊在不同的有丝分裂阶段显示出许多3-4微米的细胞核，并且内层较厚，对鬼笔环肽具有很强的亲和力。成熟的孢子囊显示出许多5-12微米的细胞壁内生孢子，每个内含2-4微米直径的核。鬼笔环肽不与成熟的孢子囊或内生孢子的内层结合。研究还证实，在成熟的孢子囊内层有数百个核，形成了一个“发芽区”，这项研究表明，在R. seeberi生命周期中，发生了同步的细胞分裂，没有胞质分裂，导致成千上万个细胞分裂。另一方面，胞质分裂是一次事件，发生在中间孢子囊的最新阶段，成千上万个核的形成之后和成熟孢子囊的发展之前。

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