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首页> 外文期刊>Journal of Parasitology >COMPARISON OF THE SPIROCHETE BORRELIA BURGDORFERI S. L. ISOLATED FROM THE TICK IXODES SCAPULARIS IN SOUTHEASTERN AND NORTHEASTERN UNITED STATES
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COMPARISON OF THE SPIROCHETE BORRELIA BURGDORFERI S. L. ISOLATED FROM THE TICK IXODES SCAPULARIS IN SOUTHEASTERN AND NORTHEASTERN UNITED STATES

机译：东南和东北美国THE虫中分离出的小球囊伯氏疏螺旋体的比较

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摘要

Thirty-five Strains of the Lyme disease spirochete Borrelia burgdoferi sensu lato (B. burgdorferi s. l.) were isolated from the blacklegged tick vector Ixodes scapularis ill South Carolina. Georgia, Florida. and Rhode Island. They were characterized by PCR-restriction fragment length polymorphism (RFLP) analysis of rrf (5S)-rrl (23S) intergenic spacer amplicons. PCR-RFLP analysis indicated that the strains represented at least 3 genospecies (including a possible novel genospecies) and 4 different restriction patterns. Thirty strains belonged to the genospecies B. burgdorferi sensu stricto (B. burgdorferi s. s.), 4 southern 1 strains were identified as B. bissettii. and strain SCCH-5 front South Carolina exhibited Mse I and Dra I restriction patterns different from those of previously reported genospecies. Complete sequences of rrf-rrl intergenic spacers from 14 southeastern and northeastern strains were determined and the phylogenetic relationships of these Strains were compared. The 14 strains clustered into I Separate lineages oil the basis of sequence analysis. These results were continued by phylogenetic analysis based oil 16S rDNA sequence analysis
机译：从南卡罗莱纳州的黑腿tick载体I肩I（Ixodes scapularis）分离出三十五株莱姆病螺旋体疏螺旋体伯氏疏螺旋体（B.burgdorferi s.l.）。佛罗里达乔治亚州。和罗德岛。通过rrf（5S）-rrl（23S）基因间隔子扩增子的PCR限制性片段长度多态性（RFLP）分析来表征它们。 PCR-RFLP分析表明，该菌株代表了至少3种基因（包括可能的新基因）和4种不同的限制模式。 30个菌株属于B. burgdorferi sensu stricto（B. burgdorferi s。s。）的基因种，其中4个南部1个菌株被鉴定为Bs bissettii。南卡罗来纳州的SCCH-5菌株和SCCH-5菌株表现出的Mse I和Dra I限制模式与以前报道的基因种不同。确定了来自东南和东北的14个菌株的rrf-rrl基因间隔子的完整序列，并比较了这些菌株的系统发生关系。在序列分析的基础上，将14个菌株聚集成I分离谱系。通过基于系统发育分析的油16S rDNA序列分析来继续这些结果

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