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首页> 外文期刊>Journal of Shellfish Research >The MIST (TM) shipable cell bioassay kits for PSP: An alternative to themouse bioassay
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The MIST (TM) shipable cell bioassay kits for PSP: An alternative to themouse bioassay

机译：用于PSP的MIST（TM）可运输细胞生物测定试剂盒：小鼠生物测定的替代方法

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摘要

A shipable cell bioassay kit called MIST(TM) (Maritime In Vitro Shellfish Test) has been developed by Jillett Biotek Ltd. for Paralytic Shellfish Poisoning (PSP). This technology is a cost-effective alternative to the mouse bioassay, and is 20 times more sensitive than the mouse. The kits provide precultured cells and premixed reagents, eliminating the need for tissue culture facilities or expertise and are robust enough to be performed in the field or on a ship. They are available in three formats: fully quantitative, semiquantitative, and a qualitative (yeso) version, called Mini-MIST(TM). The shelf life of the quantitative MIST(TM) Kit is 3 weeks, although this can be extended by freezing the cells to -100 degrees C. The kits have been successfully shipped from Canada to as far away as Australia and New Zealand. The MIST(TM) technology has been used to determine total PSP toxicity in acid extracts of phytoplankton, lobster hepatopancreas, mussels, scallops, clams, cyanobacteria, and processed food. It has also been used to, determine toxicity in phytoplankton samples. Results of parallel trials with the cell bioassay and the mouse bioassay are summarized.
机译：Jillett Biotek Ltd.已开发出一种可运输的细胞生物测定试剂盒，称为MIST™（海上体外贝类测试），用于麻痹性贝类中毒（PSP）。这项技术是鼠标生物测定的一种经济高效的替代方法，灵敏度比小鼠高20倍。该套件提供了预培养的细胞和预混合的试剂，无需组织培养设施或专业知识，并且足够坚固，可以在野外或在船上执行。它们以三种格式提供：完全定量，半定量和定性（是/否）版本，称为Mini-MIST（TM）。 MIST™定量试剂盒的保质期为3周，尽管可以通过将细胞冷冻至-100摄氏度来延长其寿命。该试剂盒已成功地从加拿大运至遥远的澳大利亚和新西兰。 MIST（TM）技术已用于确定浮游植物，龙虾肝胰腺，贻贝，扇贝，蛤，蓝藻和加工食品的酸性提取物中的总PSP毒性。它也已用于确定浮游植物样品的毒性。总结了与细胞生物测定法和小鼠生物测定法平行试验的结果。

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