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首页> 外文期刊>Journal of separation science. >HPLC-ESI-MS analysis of oral human fluids reveals that gingival crevicular fluid is the main source of oral thymosins beta(4) and beta(10)
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HPLC-ESI-MS analysis of oral human fluids reveals that gingival crevicular fluid is the main source of oral thymosins beta(4) and beta(10)

机译：口服人体液体的HPLC-ESI-MS分析表明，龈沟液是口服胸腺素beta（4）和beta（10）的主要来源

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摘要

Thymosin beta(4) (T beta(4)), its sulfoxide, and thymosin beta(10) (T beta(10)) were detected in human saliva and identified by different strategies based on RP HPLC coupled to electrospray multidimensional IT MS. T beta(4) was almost always detected in whole saliva, its sulfoxide sporadically, T beta(10) rarely. T beta(4) was undetectable in parotid saliva and less concentrated in submandibular/sublingual saliva than in whole saliva. Analysis of gingival crevicular fluid revealed high relative amounts of T beta(4), T beta(4) sulfoxide, and T beta(10) in all the samples. T beta(4) mean concentration was 200 times higher in crevicular fluid (20 mu mol/L, N = 9) than in whole saliva (0.1 mu mol/L, N = 9). Crevicular fluid concentration of T beta(4) (ca. 5% represented by its sulfoxide) and beta(10) significantly correlated (r = 0.856; N = 9), and their ratio was about 5. A significant correlation was also observed between T beta(4) concentrations in whole saliva and gingival crevicular fluid (r = 0,738; N = 9). Immunohistochemical analysis of the major salivary glands showed that immunoreactivity for T beta(4) is restricted to ductal cells, with minor degree of focal positivity in some acinar cells. On the whole, results indicate that gingival sulcus is a main, although not the sole, source for oral T beta(4) and T beta(10).
机译：在人唾液中检测到胸腺素beta（4）（T beta（4）），其亚砜和胸腺素beta（10）（T beta（10）），并通过基于RP HPLC和电喷雾多维IT MS的不同策略进行鉴定。 T beta（4）几乎总是在整个唾液中检测到，偶有其亚砜，T beta（10）很少。在腮腺唾液中检测不到T beta（4），在颌下/舌下唾液中的Tβ（4）浓度低于整个唾液。牙龈沟液的分析显示，所有样品中T beta（4），T beta（4）亚砜和T beta（10）的相对含量较高。 Tβ（4）的平均浓度在唾液中（20μmol/ L，N = 9）比整个唾液（0.1μmol/ L，N = 9）高200倍。 T beta（4）（约占其亚砜的5％）和beta（10）的缝液浓度显着相关（r = 0.856; N = 9），并且它们的比率约为5。整个唾液和龈沟液中的T beta（4）浓度（r = 0738; N = 9）。对主要唾液腺的免疫组织化学分析表明，T beta（4）的免疫反应性仅限于导管细胞，某些腺泡细胞中的局灶性阳性程度较小。总体而言，结果表明，龈沟是口服T beta（4）和T beta（10）的主要来源，尽管不是唯一的来源。

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