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首页> 外文期刊>Journal of Sol-Gel Science and Technology >Structural Stability of Azurin Encapsulated in Sol-Gel Glasses:A Fluorometric Study
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Structural Stability of Azurin Encapsulated in Sol-Gel Glasses:A Fluorometric Study

机译：溶胶-凝胶玻璃中天青素的结构稳定性：荧光法研究

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摘要

In this study we investigated the structural features of azurin,a blue copper-containing enzyme,upon encapsulation in tetramethoxysilane derived sol-gel glasses.Fluorescence spectroscopy revealed that gelation of inorganic networks does not affect the protein tertiary structure and only after two months solvent phase loss altered protein stability.In case of organically modified sol-gel matrices,the protein stability was reduced after encapsulation into hosts modified by adding 3-Mercaptopropyl-trimethoxysilane,3-Glycidyloxypropyl-trimethoxysilane and Trimethoxy octylsilane,while it was found to be enhanced in networks doped with 3-Trimethoxysilyl-propyl methacrylate and 3-Aminopropyl-trimethoxysilane.In order to better investigate the effects of silica glasses on azurin stability,unfolding experiments of the protein,in solution or entrapped,were also performed in the presence of both methanol and guanidinium hydrochloride(GdHCl).Our results suggest that the matrix protects azurin against the aggregation induced by alcohol,and that the free energy change value upon unfolding by GdHCl was lower than the value calculated for azurin in solution and was dependent on the surface chemistry of silica matrix.
机译：在这项研究中，我们研究了天青素的结构特征，天青素是一种蓝色的含铜酶，在四甲氧基硅烷衍生的溶胶-凝胶玻璃中封装后。荧光光谱表明，无机网络的凝胶化不会影响蛋白质的三级结构，仅在溶剂相两个月后在有机修饰的溶胶-凝胶基质的情况下，将其包封到通过加入3-巯基丙基三甲氧基硅烷，3-缩水甘油基氧基丙基-三甲氧基硅烷和三甲氧基辛基硅烷修饰的宿主中后，蛋白质稳定性降低，但发现在为了更好地研究二氧化硅玻璃对天青蛋白稳定性的影响，还对蛋白质在溶液中或在溶液中或被夹带的展开实验进行了研究，以更好地研究硅胶玻璃对天青蛋白稳定性的影响。和盐酸胍（GdHCl）。首先是由醇引起的聚集，GdHCl展开时的自由能变化值低于溶液中天青蛋白的计算值，并且取决于二氧化硅基质的表面化学性质。

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