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首页> 外文期刊>Journal of Ethnopharmacology: An Interdisciplinary Journal Devoted to Bioscientific Research on Indigenous Drugs >Effects of Schisandra chinensis Baillon (Schizandraceae) on lipopolysaccharide induced lung inflammation in mice
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Effects of Schisandra chinensis Baillon (Schizandraceae) on lipopolysaccharide induced lung inflammation in mice

机译：五味子五味子（Schizandraceae）对脂多糖诱导的小鼠肺部炎症的影响

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Ethnopharmacological relevance: Schisandra chinensis Baillon (Sc), an anti-inflammatory herb that has been used in traditional Chinese medicine for thousands of years, is frequently used to treat upper respiratory tract infections. Aim of the study: This study was conducted to evaluate the ability of a water extract of Sc to prevent airway inflammation both in vitro and in vivo. Materials and methods: Human lung alveolar epithelial-derived A549 cells were stimulated with to interleukin-1β, tumor necrosis factor-α, and interferon-γ (IL-1β, TNF-α, and INF-γ; cytokine mixture; CM) and treated with Sc extracts. They were then evaluated using nitric oxide (NO), IL-8 and monocyte chemotactic protein-1 (MCP-1) secretions. In the in vivo study, BALB/c mice were challenged with lipopolysaccharide (LPS) to induce acute airway inflammation. After this challenge, the mice were treated with Sc extracts (10, 50 and 100 mg/kg) by oral administration, and inflammatory cells in the bronchoalveolar lavage (BAL) fluid were counted. IL-6 and TNF-α secretions were measured using an enzyme-linked immunosorbent assay. Lung tissues of the LPS treated mice were prepared and stained with hematoxylin and eosin (HE) for histological examination. Results: In the A549 cells, Sc extracts dose-dependently and significantly inhibited CM-induced NO production and reduced IL-8 and MCP-1 secretions. Sc extracts efficiently suppressed neutrophil and macrophage infiltrations of lung tissues and increased IL-6 and TNF-α levels in BAL fluid in LPS-instilled BALB/c mice. In addition, Sc extracts treatment inhibited pathologic progress in the lung tissues, as confirmed by H&E staining. These findings indicate that Sc extracts could be potentially useful for the treatment of acute lung inflammation and acute lung injury.
机译：民族药理学联系：五味子五味子（一种抗炎药）已在中药中使用了数千年，经常被用于治疗上呼吸道感染。研究目的：进行本研究以评估Sc的水提取物在体外和体内预防气道炎症的能力。材料和方法：用白细胞介素-1β，肿瘤坏死因子-α和干扰素-γ（IL-1β，TNF-α和INF-γ;细胞因子混合物; CM）刺激人肺泡上皮来源的A549细胞和用Sc提取物处理。然后使用一氧化氮（NO），IL-8和单核细胞趋化蛋白1（MCP-1）分泌物对其进行评估。在体内研究中，用脂多糖（LPS）攻击BALB / c小鼠以诱导急性气道炎症。攻击后，通过口服给予Sc提取物（10、50和100 mg / kg）处理小鼠，并计数支气管肺泡灌洗液（BAL）中的炎症细胞。使用酶联免疫吸附测定法测量IL-6和TNF-α分泌。制备经LPS处理的小鼠的肺组织，并用苏木精和曙红（HE）染色以进行组织学检查。结果：在A549细胞中，Sc呈剂量依赖性提取，并显着抑制CM诱导的NO生成并减少IL-8和MCP-1分泌。 Sc提取物可有效抑制LPS注入的BALB / c小鼠的肺组织中性粒细胞和巨噬细胞浸润，并增加BAL液中IL-6和TNF-α的水平。此外，H＆E染色证实，Sc提取物的治疗抑制了肺组织的病理进展。这些发现表明，Sc提取物可能对治疗急性肺部炎症和急性肺损伤有用。

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