...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Structural Biology >Serial block-face scanning electron microscopy for three-dimensional analysis of morphological changes in mitochondria regulated by Cdc48p/p97 ATPase
【24h】

Serial block-face scanning electron microscopy for three-dimensional analysis of morphological changes in mitochondria regulated by Cdc48p/p97 ATPase

机译：连续块面扫描电子显微镜对Cdc48p / p97 ATPase调控的线粒体形态变化进行三维分析

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Cdc48p is a highly conserved cytosolic AAA chaperone that is involved in a wide range of cellular processes. It consists of two ATPase domains (D1 and D2), with regulatory regions at the N- and C-terminals. We have recently shown that Cdc48p regulates mitochondrial morphology, in that a loss of the ATPase activity or positive cooperativity in the D2 domain leads to severe fragmentations and aggregations of mitochondria in the cytoplasm. We have now used serial block-face scanning electron microscopy (SBF-SEM), an advanced three-dimensional (3D) electron microscopic technique to examine the structures and morphological changes of mitochondria in the yeast Saccharomyces cerevisiae. We found that mutants lacking ATPase activity of Cdc48p showed mitochondrial fragmentations and aggregations, without fusion of the outer membrane. This suggests that the ATPase activity of Cdc48p is necessary for fusion of the outer membranes of mitochondria. Our results also show that SBF-SEM has considerable advantages in morphological and quantitative studies on organelles and intracellular structures in entire cells
机译：Cdc48p是高度保守的胞质AAA分子伴侣，涉及许多细胞过程。它由两个ATPase域（D1和D2）组成，在N和C端带有调节区。我们最近显示，Cdc48p调节线粒体形态，因为ATPase活性或D2结构域中正合作性的丧失导致细胞质中线粒体的严重分裂和聚集。现在，我们已经使用了串行块面扫描电子显微镜（SBF-SEM），这是一种先进的三维（3D）电子显微镜技术，用于检查酿酒酵母中线粒体的结构和形态变化。我们发现缺少Cdc48p ATPase活性的突变体显示线粒体片段化和聚集，而没有外膜融合。这表明Cdc48p的ATPase活性对于线粒体外膜的融合是必需的。我们的结果还表明，SBF-SEM在整个细胞中细胞器和细胞内结构的形态学和定量研究中具有相当大的优势。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号