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首页> 外文期刊>Journal of the American Society for Horticultural Science >Antioxidant Enzyme Activity and Gene Expression in Response to Lead Stress in Perennial Ryegrass
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Antioxidant Enzyme Activity and Gene Expression in Response to Lead Stress in Perennial Ryegrass

机译：多年生黑麦草对铅胁迫的抗氧化酶活性和基因表达

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Lead pollution is an important issue in the world. Perennial ryegrass (Lolium perenne), as one of the widely used turfgrass and forage species, has a potential for bioremediation. The objective of this study was to investigate how antioxidant enzymes and their gene transcripts respond to Pb stress in perennial ryegrass. Ryegrass seedlings were subjected to 0, 0.5, and 3.2 mM of Pb(NO3)(2) for 7 days in a hydroponic system maintained in a greenhouse. Both root and shoot growths were inhibited by Pb compared with the control. However, contents of chlorophyll (Chl) a and total Chl were unaffected by Pb treatment. Results from this study showed a substantial increase of malondialdehyde (MDA) content in leaf tissues when perennial ryegrass was exposed to Pb at 3.2 mm. The MDA content from plants in the 0.5 trim Pb treatment was lower than the control, indicating that an effective defense mechanism existed. Circumstantial evidence came also from the content of soluble protein in 0.5 mm Pb treatment, which was not different from the control. Furthermore, the activity of catalase (CAT) increased at 0.5 mm Pb compared with the control, indicating that CAT might play an important role in scavenging reactive oxygen species (ROS). The expression profiles of eight genes encoding antioxidative enzymes were upregulated within 24 hours of Pb treatment. In conclusion, antioxidant enzymes responded to Pb at an early stage of exposure and their gene expression profiles provided more details in time courses of the activation of those systems.
机译：铅污染是世界上的重要问题。多年生黑麦草（黑麦草）作为广泛使用的草皮和牧草种类之一，具有生物修复的潜力。这项研究的目的是研究多年生黑麦草中抗氧化酶及其基因转录物对铅胁迫的反应。在维持于温室的水培系统中，对黑麦草幼苗进行0、0.5和3.2 mM的Pb（NO3）（2）处理7天。与对照相比，铅对根和芽的生长均具有抑制作用。但是，叶绿素（Chl）a和总Chl的含量不受Pb处理的影响。这项研究的结果表明，当多年生黑麦草暴露于3.2 mm的Pb时，叶片组织中的丙二醛（MDA）含量大大增加。在0.5修剪Pb处理中植物的MDA含量低于对照，表明存在有效的防御机制。周围证据也来自0.5 mm Pb处理中的可溶性蛋白含量，与对照组无差异。此外，与对照相比，过氧化氢酶（CAT）的活性在0.5 mm Pb时增加，表明CAT可能在清除活性氧（ROS）中起重要作用。铅处理后24小时内，八个编码抗氧化酶的基因的表达谱被上调。总之，抗氧化酶在暴露的早期对Pb有反应，其基因表达谱在激活这些系统的时间过程中提供了更多细节。

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