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首页> 外文期刊>Journal of Stored Products Research >Toxicity and growth inhibitory activities of methanol extract and the beta -carboline alkaloids of Peganum harmala L. against two coleopteran stored-grain pests.
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Toxicity and growth inhibitory activities of methanol extract and the beta -carboline alkaloids of Peganum harmala L. against two coleopteran stored-grain pests.

机译:甲醇提取物和香蒲的β-咔啉生物碱对两种鞘翅目储粮害虫的毒性和生长抑制活性。

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摘要

Methanol extract and the beta -carboline alkaloids were extracted from the seeds of Peganum harmala (Zygophyllaceae). Their toxicity, growth inhibitory and effects on the progeny production of Tribolium castaneum and Rhyzopertha dominica was studied. To assess any additive effects among the extracted beta -carbolines, they were tested as binary mixtures (1:1) or as a crude alkaloid fraction. All extracts exhibited a considerable adulticidal effect with increasing activities in response to increased exposure period. Using the contact toxicity bioassay, the crude beta -carboline fraction was the most effective (LC50's were 20.1 and 36.7) micro g/cm2, 48 h post-treatment against R. dominica and T. castaneum, respectively. LC50's of (harmaline+harmine), (harmaline+harmane), and methanol extract were (31.2, 39.4), (33.7, 47.2), and (39.8, 65.2) micro g/cm2, 24 h post-treatment against R. dominica and T. castaneum, respectively. At 48 h post-treatment, LC50 of (harmaline+harmine) reached 22.4 micro g/cm2 against R. dominica. When mixed with the insect's diets, toxicity of all extracts were increased with the crude alkaloidal fraction the most toxic (LC50's were 7.8 and 14.7) mg/kg grains, 48 h post exposure against R. dominica and T. castaneum, respectively. When the 2nd instar larvae were fed sub-lethal doses-treated grains, development and F1 progeny of both insects were significantly affected (P<=0.001). At 3.5 mg/kg grains of the crude alkaloidal extract, percentages of malformed larvae and pupae of T. castaneum were 19.7 and 33.4%, respectively. In this case, a total life span of 81.3 days was recorded for the treated individuals compared to 44.2 for the control. A reduction in the adult progeny of 56.9, 44.0 and 43.6% was obtained with 3.5 mg/kg of the crude alkaloids, (harmaline+harmine) and methanol extract, respectively. Meanwhile, the reduction in adult progeny of R. dominica reached 79.2% with the same concentration of the crude alkaloid extract.
机译:从甜叶天竺葵(Zygophyllaceae)的种子中提取甲醇提取物和β-咔啉生物碱。研究了它们的毒性,生长抑制作用以及对cast藜和百里香的子代生产的影响。为了评估提取的β-咔啉之间的任何加成效应,将它们作为二元混合物(1:1)或作为粗生物碱馏分进行测试。所有提取物均表现出相当大的杀螨效果,并随着暴露时间的增加而增加活性。使用接触毒性生物分析法,在处理48小时后,粗制的β-咔啉馏分最有效(LC 50's 为20.1和36.7)micro g / cm 2 R。多米尼克和 T。栗。 (harmaline + harmine),(harmaline + harmane)和甲醇提取物的LC 50's(sub)(31.2,39.4),(33.7,47.2)和(39.8,65.2)micro g / cm 2 ,针对 R的治疗后24小时。多米尼克和 T。栗。在处理后48小时,(harmaline + harmine)的LC 50 对 R达到22.4 micro g / cm 2 。多米尼加。当与昆虫的饮食混合时,所有生物提取物的毒性均增加,其中粗制生物碱部分毒性最大(LC 50's 分别为7.8和14.7)mg / kg谷物,暴露于 R后48小时。多米尼克和 T。栗子。当给第二龄幼虫喂食亚致死剂量的谷物时,两种昆虫的发育和F1子代均受到显着影响( P <0.001)。粗生物碱提取物的籽粒含量为3.5 mg / kg时,畸形幼虫和T的百分比。栗皮分别为19.7%和33.4%。在这种情况下,治疗个体的总寿命为81.3天,而对照组为44.2天。用3.5 mg / kg的粗生物碱(harmaline + harmine)和甲醇提取物,分别可将成年子代减少56.9、44.0和43.6%。同时,成人后代的减少。在相同浓度的生物碱提取物中多米尼加达到79.2%。

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