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首页> 外文期刊>Journal of Pharmacy and Pharmacology >Effect of berberine on proliferation, biosynthesis of macromolecules, cell cycle and induction of intercalation with DNA, dsDNA damage and apoptosis in Ehrlich ascites carcinoma cells.
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Effect of berberine on proliferation, biosynthesis of macromolecules, cell cycle and induction of intercalation with DNA, dsDNA damage and apoptosis in Ehrlich ascites carcinoma cells.

机译：小ber碱对艾氏腹水癌细胞增殖，大分子生物合成，细胞周期及DNA嵌入，dsDNA损伤和凋亡的影响。

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摘要

Our primary aim was to study berberine, a potential anti-cancer drug, for its cytotoxic and antiproliferative activity in-vitro using Ehrlich ascites carcinoma (EAC) cells. Cytotoxicity was measured by the growth inhibition assay. We investigated the effect of berberine on the biosynthesis of macro-molecules (DNA, RNA, proteins), cell cycle effects and induction of dsDNA damage and apoptosis in berberine-treated EAC cells. Our results showed that berberine acts cytotoxically on EAC cells. The cytotoxicity was directly concentration and time dependent. The highest cytotoxic concentrations (100 and 50 microg mL(-1)) induced intercalation of berberine with DNA, formation of dsDNA breaks, inhibition of DNA synthesis and death of EAC cells. A concentration of 10 mug mL(-1) induced clear apoptotic cell death, which was followed by inhibition of protein synthesis.
机译：我们的主要目标是使用Ehrlich腹水癌（EAC）细胞研究小碱（一种潜在的抗癌药）的体外细胞毒性和抗增殖活性。通过生长抑制测定法测量细胞毒性。我们研究了小ber碱对大分子（DNA，RNA，蛋白质）的生物合成，细胞周期效应以及小DNA碱处理的EAC细胞中dsDNA损伤和凋亡诱导的影响。我们的结果表明，小ber碱对EAC细胞具有细胞毒性作用。细胞毒性直接与浓度和时间有关。最高的细胞毒性浓度（100和50微克mL（-1））诱导小ber碱与DNA的嵌入，dsDNA断裂的形成，DNA合成的抑制和EAC细胞的死亡。浓度为10杯mL（-1）诱导明显的凋亡细胞死亡，然后抑制蛋白质合成。

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