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首页> 外文期刊>Clinical neuropathology >FISH-based detection of 1p 19q codeletion in oligodendroglial tumors: procedures and protocols for neuropathological practice - a publication under the auspices of the Research Committee of the European Confederation of Neuropathological Societies (
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FISH-based detection of 1p 19q codeletion in oligodendroglial tumors: procedures and protocols for neuropathological practice - a publication under the auspices of the Research Committee of the European Confederation of Neuropathological Societies (

机译：基于FISH的少突神经胶质瘤中1p 19q缺失的检测：神经病理学实践的程序和协议-由欧洲神经病理学会联合会研究委员会主持出版的出版物（

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摘要

The codeletion of chromosomal arms 1p 19q is a characteristic and early genetic event in oligodendroglial tumors, that is associated with a better prognosis and enhanced response to therapy. Over the last years, the increasing clinical demand to determine the 1p 19q status has led to the implementation of its testing in many neuropathology laboratories. Several different methods for 1p 19q testing are available: PCR-based loss of heterozygosity analysis, multiplex ligation-dependent probe amplification, array comparative genomic hybridization, and fluorescence in situ hybridization (FISH). Herein, we focus and critically discuss the latter method because a detailed description of procedures and protocols for FISH-based 1p 19q testing in practice is lacking. We present a practical approach to the FISH-based assessment of the 1p 19q status in oligodendroglial tumors, from commonly used locus-specific probes and technical protocols to the neuropathological interpretation of results. Thereby, we aim to facilitate the implementation of FISH-based 1p 19q testing for clinical purposes in standard neuropathology laboratories without special focus on brain tumor research.
机译：染色体臂1p 19q的密码缺失是少突胶质细胞瘤的特征性早期遗传事件，与更好的预后和对治疗的增强反应有关。在过去的几年中，确定1p 19q状态的临床需求不断增长，导致许多神经病理学实验室实施了其测试。可以使用几种不同的方法进行1p 19q测试：基于PCR的杂合性丧失分析，依赖多重连接的探针扩增，阵列比较基因组杂交和荧光原位杂交（FISH）。在本文中，由于缺乏对基于FISH的1p 19q测试的过程和协议的详细描述，因此我们集中讨论了后一种方法。我们提供了一种实用的方法来评估少突胶质细胞瘤中1p 19q状态的基于FISH的评估，从常用的基因座特异性探针和技术方案到结果的神经病理学解释。因此，我们旨在促进在标准神经病理学实验室中基于临床目的的基于FISH的1p 19q测试的实施，而无需特别关注脑肿瘤研究。

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