首页> 外文期刊>Journal of the Korean Society for Applied Biological Chemistry >New Enzymatic Time-temperature Integrator (TTI) Using Porcine Esterase for Monitoring Food Quality
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New Enzymatic Time-temperature Integrator (TTI) Using Porcine Esterase for Monitoring Food Quality

机译:使用猪酯酶的新型酶促时温积分仪(TTI)监测食品质量

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An enzymatic time-temperature integrator (TTI) system based on the reaction between porcine esterase and tripropionin, which causes a pH change, was developed. A pH decline in the esterase-based TTI system changed the color of the pH indicator from blue to light green. After calculating the response rate constant by measuring color changes in the TIT under different temperature conditions, an Arrhenius plot was prepared, and activation energy was estimated. The activation energy of the TTI measured at isothermal conditions (5, 10, 15, 20, and 30 degrees C) was 53.6-54.6 kJ/mol. In addition, the TB endpoint could be adjusted by modifying the amount of esterase consumed to fit different shelf lives of food. The results showed that the new esterase-based TTI could be used to indicate the time-temperature history of food.
机译:开发了一种基于猪酯酶和三丙酸酯之间的反应(会引起pH值变化)的酶促时间-温度积分器(TTI)系统。基于酯酶的TTI系统的pH下降使pH指示剂的颜色从蓝色变为浅绿色。通过测量不同温度条件下TIT的颜色变化来计算响应速率常数后,准备了Arrhenius图,并估算了活化能。在等温条件下(5、10、15、20和30摄氏度)测得的TTI活化能为53.6-54.6 kJ / mol。另外,可以通过改变所消耗的酯酶的量来适应结核的终点,以适应食品的不同保质期。结果表明,新的基于酯酶的TTI可用于指示食品的时间-温度历史。

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