• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of the Korean Society for Applied Biological Chemistry >Artificial Induction of Cadmium Tolerance and its Further Enhancement via Heterologous Co-expression of SpHMT1 and AtPCS1 in the Yeast Cells
【24h】

Artificial Induction of Cadmium Tolerance and its Further Enhancement via Heterologous Co-expression of SpHMT1 and AtPCS1 in the Yeast Cells

机译：人工诱导的镉耐受性及其通过酵母细胞中SpHMT1和AtPCS1的异源共表达进一步增强。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Microorganisms can be used in the bioremediation of heavy metals. It is desirable if the microorganisms show a strong tolerance as well as the ability to accumulate (or biosorb) heavy metals. Yeast is well known for having a high capacity of biosorption of heavy metals. Therefore, the present study focused on developing a yeast mutant that has a strong tolerance to cadmium (Cd), a representative toxic metal. The Cd-resistant yeast mutant (CdR) was induced and isolated by growing yeast cells in media containing Cd and gradually increasing the concentration until reaching a possible maximum of 20 mM CdCl2, to which the cells adapted and survived. CdR cells showed stronger tolerance to Cd-induced stress than the control cells. To obtain even higher Cd tolerance in the yeast cells, both Schizosaccharomyces pombe heavy metal tolerance factor 1 (SpHMT1) and Arabidopsis thaliana phytochelatin synthase (AtPCS1) genes were introduced into the CdR cells to be expressed simultaneously. The transformed CdR cells showed higher Cd tolerance than the untransfonned CdR cells.
机译：微生物可用于重金属的生物修复。如果微生物显示出强的耐受性以及积累（或生物吸附）重金属的能力，则是合乎需要的。酵母以对重金属的高生物吸附能力而闻名。因此，本研究着重于开发对具有代表性的有毒金属镉（Cd）具有强耐受性的酵母突变体。通过使酵母细胞在含Cd的培养基中生长并逐渐增加其浓度直至达到可能的最大20 mM CdCl2来诱导和分离抗Cd的酵母突变体（CdR），细胞可以适应并存活。 CdR细胞比对照细胞对Cd诱导的应激表现出更强的耐受性。为了在酵母细胞中获得更高的Cd耐受性，将粟酒裂殖酵母重金属耐受因子1（SpHMT1）和拟南芥植物螯合酶合酶（AtPCS1）基因都引入CdR细胞中，以便同时表达。转化的CdR细胞显示出比未转化的CdR细胞更高的Cd耐受性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号