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首页> 外文期刊>Journal of the Korean Society for Applied Biological Chemistry >Purification and characterization of two bacteriocins from Lactobacillus brevis BK11 and Enterococcus faecalis BK61 showing anti-Helicobacter pylori activity
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Purification and characterization of two bacteriocins from Lactobacillus brevis BK11 and Enterococcus faecalis BK61 showing anti-Helicobacter pylori activity

机译：短乳杆菌BK11和粪肠球菌BK61的两种细菌素的纯化和鉴定显示抗幽门螺杆菌活性

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摘要

The aim of this study was to investigate the antimicrobial effects of purified bacteriocins isolated from Lactobacillus brevis BK11 and Enterococcus faecalis BK61 on Helicobacter pylori. After the final purification step, the molecular weights of the purified bacteriocins from L. brevis BK11 and E. faecalis BK61 were estimated to be approximately 6.5 and 4.5 kDa, respectively. Significant decrease in the antimicrobial activity of these bacteriocins was observed when they were treated with several proteolytic enzymes. However, their antimicrobial activity was stable over long periods of storage and a wide range of pH and was relatively heat resistant. The inhibitory spectrum of the bacteriocin produced by L. brevis BK11 strain was quite narrow, whereas the bacteriocin produced from E. faecalis BK61 strain exhibited a broad antimicrobial spectrum against various foodborne pathogens such as Listeria monocytogenes, Escherichia coli O157, and Salmonella enteritidis. Treatment of H. pylori with these bacteriocins significantly reduced the number of cells of the pathogen found adhering to the monolayers of cultured human gastric adenocarcinoma epithelial cell line (p < 0.05). Furthermore, the urease activity of adherent H. pylori after treatment with the bacteriocins was lower than that for the control group, but there appeared to be no significant difference between the competition and displacement groups (p > 0.05).
机译：这项研究的目的是调查从短乳杆菌BK11和粪肠球菌BK61分离出的纯化细菌素对幽门螺杆菌的抗菌作用。在最后的纯化步骤之后，估计来自短乳杆菌BK11和粪肠球菌BK61的纯化细菌素的分子量分别约为6.5和4.5 kDa。这些细菌素经几种蛋白水解酶处理后，其抗菌活性显着降低。然而，它们的抗微生物活性在长期储存和宽pH范围内是稳定的，并且是相对耐热的。短乳杆菌BK11菌株产生的细菌素的抑制谱很窄，而粪肠球菌BK61菌株产生的细菌素对各种食源性病原体如单核细胞增生性李斯特菌，大肠杆菌O157和肠炎沙门氏菌表现出广泛的抗菌谱。用这些细菌素处理幽门螺杆菌可显着减少粘附在培养的人胃腺癌上皮细胞系单层上的病原体的细胞数量（p <0.05）。此外，细菌素处理后粘附的幽门螺杆菌的脲酶活性低于对照组，但是竞争组和置换组之间似乎没有显着差异（p> 0.05）。

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