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首页> 外文期刊>Journal of the Korean Society for Applied Biological Chemistry >Isolation of Sporidiobolus ruineniae CO-3 and Characterization of Its Extracellular Protease
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Isolation of Sporidiobolus ruineniae CO-3 and Characterization of Its Extracellular Protease

机译：螺孢霉菌CO-3的分离及其胞外蛋白酶的鉴定

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摘要

An extracellular protease-producing yeast strain, designated as CO-3, was isolated from fermented tea. The cells were spherical- to ovoid-shaped and 6.8-7.4x7.4-9.1 urn in size. Optimal growth conditions were 25-30°C and pH of 5.0-6.0. The isolate was able to grow in up to 4%(w/v) NaCl and 5%(v/v) ethanol. This strain was identified as Sporidiobolus ruineniae based on the internal transcribed spacer regions including 5.8S rDNA sequence and partial D1/D2 domain of 26S rDNA sequence analysis. Optimalactivity conditions of the crude protease fraction of S. ruineniae CO-3 were pH of 7.0 at 50°C. Protease production reached maximum when 1.0%(w/v) xylose, 1.0%(w/v) yeast extract, and 0.3%(w/v) K_2HPO_4 were used as the sole sources of carbon, nitrogen,and mineral, respectively.
机译：从发酵茶中分离出一种称为CO-3的胞外蛋白酶生产酵母菌株。细胞为球形至卵形，大小为6.8-7.4x7.4-9.1。最佳生长条件为25-30°C，pH为5.0-6.0。分离物能够在高达4％（w / v）的NaCl和5％（v / v）的乙醇中生长。根据内部转录的间隔区（包括5.8S rDNA序列和26S rDNA序列分析的D1 / D2部分结构域），将该菌株鉴定为孢霉。鼠李糖单胞菌CO-3的粗蛋白酶级分的最佳活性条件是在50℃下pH为7.0。当分别使用1.0％（w / v）木糖，1.0％（w / v）酵母提取物和0.3％（w / v）K_2HPO_4作为碳，氮和矿物质的唯一来源时，蛋白酶的产量达到最大值。

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