首页> 外文期刊>Journal of the Korean Society for Applied Biological Chemistry >Molecular Identification and Characterization of Pseudomonas sp NCCP-407 for Phenol Degradation Isolated From Industrial Waste
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Molecular Identification and Characterization of Pseudomonas sp NCCP-407 for Phenol Degradation Isolated From Industrial Waste

机译:假单胞菌NCCP-407用于工业废水中苯酚降解的分子鉴定和表征

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摘要

Phenol is a toxic pollutant found in effluent of numerous industries and its elimination is a foremost challenge. The utilization of bacteria plays a crucial role in phenol bioremediation. For isolation of phenol degrading bacteria, sample was collected from industrial waste and enriched in mineral salt medium (MSM) contained 300 mg/L phenol. The strain was identified based on 16S rRNA gene analysis as Pseudomonas species and the phylogenetic analysis affiliated the strain with Pseudomonas monteilii (AF064458) as the most closely related species. Phenol tolerance of the strain in MSM supplemented with various concentrations of phenol indicates that the strain NCCP-407 can grow best at 750 mg L-1 phenol. The strain showed complete degradation of 750 mg L-1 phenol in 56 hours when supplement as a sole source of carbon and energy with the average degradation rate of 28 mg L-1 h(-1). The doubling time was recorded approximately as 12.49 h(-1). The present study suggests that this strain is efficient in phenol degradation and can be used in treatment of wastewater containing phenol
机译:苯酚是在许多工业废水中发现的有毒污染物,要消除苯酚是最重要的挑战。细菌的利用在苯酚生物修复中起着至关重要的作用。为了分离降解苯酚的细菌,从工业废料中收集样品,并富集含有300 mg / L苯酚的矿物盐培养基(MSM)。基于16S rRNA基因分析,将该菌株鉴定为假单胞菌属,并且系统发育分析将该菌株与最相关的物种为蒙特氏假单胞菌(AF064458)。 MSM菌株中添加了各种浓度的苯酚的菌株对苯酚的耐受性表明,NCCP-407菌株在750 mg L-1苯酚下生长最佳。当补充作为唯一的碳和能量来源时,菌株在56小时内可完全降解750 mg L-1苯酚,平均降解速率为28 mg L-1 h(-1)。记录的倍增时间约为12.49 h(-1)。本研究表明该菌株可有效降解苯酚,可用于处理含苯酚的废水。

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