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首页> 外文期刊>Journal of the Society for Gynecologic Investigation >Estrogen receptor-beta is the predominant estrogen receptor subtype in normal human synovia.
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Estrogen receptor-beta is the predominant estrogen receptor subtype in normal human synovia.

机译：在正常人滑膜中，雌激素受体-β是主要的雌激素受体亚型。

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摘要

OBJECTIVE: Joint pain increases after menopause with more than 50% of woman suffering from arthralgies. Since pain and inflammation of joints originate from synovial tissue, we aimed to discover whether estrogen receptors are present in the human synovia. METHODS: This in vitro study was performed on samples of human synovial tissue, obtained from pre- (n = 8) and postmenopausal woman (n = 11) and men (n = 5) following surgery due to traumatic lesions. Fresh synovial tissue specimens were assessed for the localization as well as the presence of estrogen receptor-alpha (ER alpha) and estrogen receptor-beta (ER beta) by means of immunohistochemistry, as well as Western blot and reverse transcriptase-polymerase chain reaction (RT-PCR), respectively. RESULTS: ER beta protein and mRNA were found to be equally and highly expressed in synovial stroma and lining cells of all explants independent of sex or menopausal status. In contrast, weak ER alpha staining was localized in the synovial lining cells in only three of 24 explants. ER alpha protein was found to be weakly expressed in three of ten explants. ER alpha mRNA was found with highly variable amounts in seven of ten explants. CONCLUSION: In view of our observation that ER beta but not ER alpha is expressed regularly in normal human synovia in high amounts, we propose that estrogen could play a significant role in synovial membrane function in women and men, operating preferably via the ER beta isoform.
机译：目的：绝经后关节疼痛加剧，超过50％的女性患有关节病。由于关节的疼痛和炎症源自滑膜组织，因此我们旨在发现人类滑膜中是否存在雌激素受体。方法：这项体外研究是对人体滑膜组织样本进行的，该样本取自因创伤而手术后的绝经前（n = 8）和绝经后妇女（n = 11）和男性（n = 5）。通过免疫组织化学，Western印迹和逆转录酶-聚合酶链反应（US），评估新鲜滑膜组织标本的定位以及雌激素受体-α（ER alpha）和雌激素受体-β（ER beta）的存在（ RT-PCR）。结果：发现ERβ蛋白和mRNA在所有外植体的滑膜基质和内衬细胞中均等且高表达，而与性别或绝经状态无关。相反，在24个外植体中只有3个外植体的滑膜衬里细胞中存在弱的ERα染色。发现ERα蛋白在十个外植体中的三个中弱表达。在十个外植体中的七个中发现了ERαmRNA的含量变化很大。结论：鉴于我们观察到正常人滑膜中ERβ而不是ERα在正常人中大量表达，我们建议雌激素在男女滑膜功能中起重要作用，最好通过ERβ同工型进行操作。

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