Utilisation of cupric engulfment by Escherichia coli for PcoC (a superoxide dismutase) induction

Shreya Bhattacharya; Santanu Roy; Rini Roy

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Utilisation of cupric engulfment by Escherichia coli for PcoC (a superoxide dismutase) induction

机译：大肠杆菌利用铜吞噬诱导PcoC（超氧化物歧化酶）

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Cu has been used as an inducer of PcoC (SOD) in E. coli to screen the time for achieving maximum activity. This SOD can be isolated from bacterial system and purified to serve as a source for use in medicines for humans since SOD proteins of humans and E. coli share significant sequence similarity. Copper engulfment capacity of E. coli has been utilised to induce PcoC (SOD) which has been measured by estimating the activity of the two proteins, PcoA and PcoC (Pco stands for plasmid borne copper resistance operon) with variation in concentration of Cu~(II) in the medium and duration of incubation. Cu~(II) engulfment capacity of E. coli through liposome is greater in case of PcoC than PcoA.

机译：铜已被用作大肠杆菌中PcoC（SOD）的诱导剂，以筛选获得最大活性的时间。由于人与大肠杆菌的SOD蛋白具有明显的序列相似性，因此可以从细菌系统中分离出该SOD，并进行纯化以用作人类药物的来源。大肠杆菌的铜吞噬能力已被用于诱导PcoC（SOD），这是通过估算两种蛋白质PcoA和PcoC（Pco代表质粒携带的铜抗性操纵子）的活性随Cu〜（浓度）的变化而测量的。二）在培养基中和持续时间。在PcoC的情况下，大肠杆菌通过脂质体的Cu〜（II）吞噬能力大于PcoA。

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《Journal of the Indian Chemical Society》 |2010年第8期|共5页
作者
Shreya Bhattacharya; Santanu Roy; Rini Roy;
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正文语种 eng
中图分类化学;
关键词
E. coli; PcoC; cupric engulfment; induction; PcoA.;

机译：大肠杆菌;PcoC;铜吞噬;诱导;PcoA。;

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1. Utilisation of cupric engulfment by Escherichia coli for PcoC (a superoxide dismutase) induction [J] . Shreya Bhattacharya, Santanu Roy, Rini Roy Journal of the Indian Chemical Society . 2010,第8期

机译：大肠杆菌利用铜吞噬诱导PcoC（超氧化物歧化酶）
2. High lipophilicity of meta Mn(III) N-alkylpyridylporphyrin-based superoxide dismutase mimics compensates for their lower antioxidant potency and makes them as effective as ortho analogues in protecting superoxide dismutase-deficient Escherichia coli [J] . Kos I., Benov L., Spasojevi? I., Journal of Medicinal Chemistry . 2009,第23期

机译：基于Mn（III）N-烷基吡啶基卟啉的超氧化物歧化酶模拟物的高亲脂性弥补了其较低的抗氧化能力，使其在保护超氧化物歧化酶缺陷型大肠杆菌方面与邻位类似物一样有效
3. Vaccination with live Escherichia coli expressing Brucella abortus Cu/Zn superoxide-dismutase: II. Induction of specific CD8+ cytotoxic lymphocytes and sensitized CD4+ IFN-gamma-producing cell. [J] . Andrews E, Salgado P, Folch H, Microbiology and Immunology . 2006,第5期

机译：用表达流产布鲁氏菌铜/锌超氧化物歧化酶的活大肠杆菌接种：II。诱导特异性CD8 +细胞毒性淋巴细胞和CD4 +IFN-γ致敏细胞。
4. Cupric Complexes of Phenolate Siderophores and Superoxide Dismutase Mimicry [C] . Daryl Giblin, Shannon Ohlemacher, Jeffrey P. Henderson, ASMS Conference on Mass Spectrometry and Allied Topics . 2014

机译：酚醛磷酸和超氧化物歧化酶模拟的铜络合物
5. Determination of cell viability of superoxide dismutase knockout Escherichia coli strains using oxidative stress assays. [D] . Beyer, Christopher Mark. 2012

机译：使用氧化应激测定法测定超氧化物歧化酶敲除大肠杆菌菌株的细胞活力。
6. Isolation of the Escherichia coli iron superoxide dismutase gene: evidence that intracellular superoxide concentration does not regulate oxygen-dependent synthesis of the manganese superoxide dismutase. [O] . C J Nettleton, C Bull, T O Baldwin, 1984

机译：大肠杆菌铁超氧化物歧化酶基因的分离：证据表明细胞内超氧化物的浓度不调节锰超氧化物歧化酶的氧依赖性合成。
7. Isolation of the Escherichia coli iron superoxide dismutase gene: evidence that intracellular superoxide concentration does not regulate oxygen-dependent synthesis of the manganese superoxide dismutase. [O] . Nettleton, C J, Bull, C, Baldwin, T O, 1984

机译：大肠杆菌铁超氧化物歧化酶基因的分离：证据表明细胞内超氧化物的浓度不调节锰超氧化物歧化酶的氧依赖性合成。
8. Induction of Superoxide Dismutase in 'Escherichia coli' by Heat Shock [R] . Privalle, C. T., Fridovich, I. 1987

机译：热休克诱导'大肠杆菌'中超氧化物歧化酶的诱导

Utilisation of cupric engulfment by Escherichia coli for PcoC (a superoxide dismutase) induction

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