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首页> 外文期刊>Journal of the Science of Food and Agriculture >Red clover (Trifolium pratense L.) isoflavones: determination of concentrations by plant stage, flower colour, plant part and cultivar.
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Red clover (Trifolium pratense L.) isoflavones: determination of concentrations by plant stage, flower colour, plant part and cultivar.

机译：红三叶草（Trifolium pratense L.）异黄酮：通过植物分期，花色，植物部位和品种确定浓度。

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摘要

BACKGROUND: Red clover (Trifolium pratense L.), a widely used fodder, has recently received considerable interest as a valuable source of isoflavones for many health-related applications. The aim of this study was to analyse the concentrations of four isoflavones, daidzein, genistein, formononetin and biochanin A, in extracts of leaves, stems, roots and different coloured flowers from red clover by high-performance liquid chromatography and to determine the antifungal activities of the extracts. RESULTS: Among greenhouse-grown samples the highest amounts of daidzein and genistein were found in petioles (0.11-0.28 and 0.30-0.54 mg g-1 respectively), while leaves were rich in formononetin and biochanin A (5.57-9.05 and 10.94-14.59 mg g-1 respectively). High formononetin concentrations were found in roots, but very little biochanin A. Among field-grown samples the highest concentrations of daidzein and genistein were detected in stems collected in August (0.24 and 0.55 mg g-1 respectively), while the highest amounts of formononetin and biochanin A were found in young leaves collected in June (7.47 and 9.69 mg g-1 respectively). Most of the extracts inhibited the growth of Drechslera teres, while the root extract showed the strongest effect against Microdochium nivale. CONCLUSION: High levels of bioactive compounds found not only in inflorescences but also in roots, leaves and stems suggest that red clover may be an inexpensive and valuable resource for many applications.
机译：背景：红三叶草（Trifolium pratense L.）是一种广泛使用的饲料，最近作为许多健康相关应用中的异黄酮的重要来源，引起了广泛的关注。这项研究的目的是通过高效液相色谱法分析红三叶草的叶子，茎，根和不同颜色的花朵中的四种异黄酮，大豆苷元，染料木黄酮，福乐菌素和生物chaninA的浓度，并确定其抗真菌活性。的提取物。结果：在温室种植的样品中，叶柄中的黄豆苷元和染料木黄酮含量最高（分别为0.11-0.28和0.30-0.54 mg g-1），而叶片中富含单果糖素和生物素A（5.57-9.05和10.94-14.59分别为mg g-1）。在根中发现较高的formononetin浓度，但几乎没有生物素A。在田间生长的样品中，在8月采集的茎中检出的大豆苷元和染料木黄酮浓度最高（分别为0.24和0.55 mg g-1），而formononetin的含量最高。在6月份收集的幼叶中分别发现了生物素和生物素A（分别为7.47和9.69 mg g-1）。大部分提取物均能抑制德雷氏菌的生长，而根提取物则显示出最强的抗小球藻的作用。结论：不仅在花序中而且在根，叶和茎中均发现高水平的生物活性化合物，这表明红三叶草对于许多应用而言可能是一种廉价且有价值的资源。

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