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首页> 外文期刊>Journal of the Science of Food and Agriculture >Polyphenol oxidase activity of two olive (Olea europaea L.) cultivars as an early criterion of Mn toxicity
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Polyphenol oxidase activity of two olive (Olea europaea L.) cultivars as an early criterion of Mn toxicity

机译：两种橄榄（Olea europaea L.）品种的多酚氧化酶活性是Mn毒性的早期指标

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BACKGROUND: The time course of polyphenol oxidase (PPO) activity in the leaves of two olive cultivars (Picual and FS-17) irrigated with nutrient solutions differing in Mn concentration (0, 2 and 1280 omol Lp#) was studied under hydroponic conditions to determine whether PPO activity could be used as an early criterion of Mn status of olive plants, and to elucidate whether genotypic differences exist between the two olive cultivars studied, concerning the effect of Mn concentration on PPO activity.RESULTS: In all the Mn treatments, PPO activity was greater in Picual than in FS-17. Under excess Mn (1280 omol Lp#), PPO activity gradually increased with time, starting from day 30 of the experiment in both cultivars, and this increase preceded the appearance of Mn toxicity symptoms. In contrast, in the other two Mn treatments (0 and 2 omol Lp#) PPO activity increased and afterwards decreased during the experiment, but the trend was not clear. In the 1280 omol Lp# treatment, PPO activity linearly increased (R = 0.8836 for Picual and 0.943 for FS-17) with the increase of Mn concentration in the leaves of both cultivars. In the 1280 omol Lp# Mn treatment, PPO activity was negatively related with Fe and Zn concentrations in the leaves, and positively in the 0 and 2 omol Lp# Mn treatments with the Ca, Mg and K concentrations.CONCLUSION: From the differential time course of PPO activity in the three Mn treatments (0, 2 and 1280 omol Lp#), it is concluded that periodic measurements of PPO activity in the leaves of the olive cultivars Picual and FS-17 can be used for the early detection of Mn toxicity (before the appearance of symptoms). Copyright
机译：背景：在水耕条件下，研究了两种不同锰浓度（0、2和1280 omol Lp＃）营养液灌溉的橄榄品种（Picual和FS-17）叶片中多酚氧化酶（PPO）活性的时程，确定是否可以将PPO活性用作橄榄植物Mn状况的早期标准，并阐明在研究的两个橄榄品种之间是否存在基因型差异，涉及Mn浓度对PPO活性的影响。结果：在所有Mn处理中， Picual中的PPO活性高于FS-17。在过量Mn（1280 omol Lp＃）下，从两个品种的实验第30天开始，PPO活性随时间逐渐增加，并且这种增加先于Mn毒性症状出现。相反，在其他两种锰处理中（0和2 omol Lp＃），在实验过程中PPO活性增加，随后降低，但趋势不明显。在1280 omol Lp＃处理中，两个品种叶片中Mn浓度的增加都使PPO活性线性增加（Ric = 0.8836（Picual）和0.943（FS-17））。在1280 omol Lp＃M​​n处理中，PPO活性与叶片中的Fe和Zn浓度呈负相关，而在0和2 omol Lp＃M​​n处理中，Ca，Mg和K浓度呈正相关。在三种锰处理（0、2和1280 omol Lp＃）中PPO活性的变化过程中，得出的结论是，定期测量橄榄品种Picual和FS-17叶片中PPO活性可用于锰的早期检测毒性（在出现症状之前）。版权

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