Theoretical analysis of the importance of recycling in measurements of protein turnover by constant infusion of a labelled amino acid.

Slevin K; Waterlow J

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Theoretical analysis of the importance of recycling in measurements of protein turnover by constant infusion of a labelled amino acid.

机译：通过循环输注标记的氨基酸对蛋白质更新测量中回收利用的重要性进行理论分析。

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In studies of whole body protein turnover, recycling of tracer from the breakdown of labelled protein is usually neglected; this neglect may introduce a significant error. A three-pool model with fast and slowly turning over protein pools has been used to calculate recycling rates over a range of sizes and turnover rates of the protein pools. Complete and approximate solutions of the equations are given. The recycling rate of 1% per hour would fit the available data on the turnover rates of human tissue proteins.

机译：在全身蛋白质更新的研究中，通常忽略了标记蛋白质分解产生的示踪剂的再循环;这种疏忽可能会导致重大错误。具有快速和缓慢周转蛋白质池的三池模型已用于计算蛋白质池在大小和周转率范围内的回收率。给出了方程的完整和近似解。每小时1％的回收率将适合人体组织蛋白质周转率的现有数据。

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《Journal of Theoretical Biology》 |2008年第1期|共5页
作者
Slevin K; Waterlow J;
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正文语种 eng
中图分类普通生物学;
关键词
Algorithms; Amino Acids; Dietary Proteins; dosage; Humans; Isotope Labeling; 算法; 氨基酸类; 膳食蛋白质类; 同位素标记; 模型; 生物学;

机译：Algorithms;Amino Acids;Dietary Proteins;dosage;Humans;Isotope Labeling;算法;氨基酸类;膳食蛋白质类;同位素标记;模型;生物学;

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1. Theoretical analysis of the importance of recycling in measurements of protein turnover by constant infusion of a labelled amino acid. [J] . Slevin K, Waterlow J Journal of Theoretical Biology . 2008,第1期

机译：通过循环输注标记的氨基酸对蛋白质更新测量中回收利用的重要性进行理论分析。
2. An analysis of errors in estimation of the rate of protein synthesis by constant infusion of a labelled amino acid [J] . P J Garlick The biochemical journal . 1978,第2期

机译：恒定输注标记氨基酸估算蛋白质合成速率时的误差分析
3. Protein turnover: Measurement of proteome dynamics by whole animal metabolic labelling with stable isotope labelled amino acids [J] . ClaydonA.J., ThomM.D., HurstJ.L., Proteomics . 2012,第8期

机译：蛋白质更新：通过用稳定同位素标记的氨基酸进行全动物代谢标记来测量蛋白质组动力学
4. HIGH-THROUGHPUT MEASUREMENT OF PROTEIN TURNOVER IN PLANTS USING STABLE ISOTOPE LABELING COUPLED WITH LC-MS/MS ANALYSIS [C] . WEN-PING CHEN, XIAO-YUAN YANG, ADRIAN D. HEGEMAN, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2009

机译：使用稳定同位素标记耦合LC-MS / MS分析植物植物中蛋白质周转的高通量测量
5. Measurements using capillary zone electrophoresis of amniotic fluid proteins and uric acid. [D] . Gao, Tao. 2007

机译：使用毛细管区带电泳对羊水蛋白和尿酸进行测量。
6. An analysis of errors in estimation of the rate of protein synthesis by constant infusion of a labelled amino acid. [O] . P J Garlick 1978

机译：通过不断输注标记的氨基酸估算蛋白质合成速率时的误差分析。
7. An analysis of errors in estimation of the rate of protein synthesis by constant infusion of a labelled amino acid. [O] . Garlick, P J 1978

机译：通过不断输注标记的氨基酸估算蛋白质合成速率时的误差分析。
8. Metabolism of nonessential N15-labeled amino acids and the measurement of human whole-body protein synthesis rates [R] . Stein, T. P., Settle, R. G., Albina, J. A., 1991

机译：非必需N15标记氨基酸的代谢和人体全身蛋白质合成率的测量

Theoretical analysis of the importance of recycling in measurements of protein turnover by constant infusion of a labelled amino acid.

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