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Built-in way to the stable isotope-labeled amino acid and its target protein, NMR structure of the protein analysis method and regioselective stable isotope-labeled fumaric acid and the production method of tartaric acid
Built-in way to the stable isotope-labeled amino acid and its target protein, NMR structure of the protein analysis method and regioselective stable isotope-labeled fumaric acid and the production method of tartaric acid
A least one of the amino acids constituting the protein, the following labeling patterns; Hydrogen of the methyl group and the other completely is deuterated other leaving a hydrogen of at least one of the methylene hydrogens in the methylene group or two or more (a) 1 In the group gem- a methyl (b) prochiral the deuterated other leaving one hydrogen, the hydrogen of the methyl group are partially deuterated are deuterated in (c) prochiral methyl groups (d) methyl group moiety aromatic ring hydrogenation To provide a stable isotope-labeled amino acids, characterized in that it has at least one of which is deuterated. By using this stable isotope-labeled amino acids, rapid and reliable analysis of the NMR spectrum of high molecular weight proteins to achieve without damaging the NMR sensitivity of the residual hydrogen nucleus, beyond the limits of conventional, deuterium substitution of the protein, It is possible to simultaneously achieve high-precision determination of the three-dimensional structure.
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