首页> 外国专利> Built-in way to its target protein with stable isotope-labeled amino acids, the production method of tartaric acid and regioselective stable isotope-labeled fumaric acid and NMR structural analysis method of protein

Built-in way to its target protein with stable isotope-labeled amino acids, the production method of tartaric acid and regioselective stable isotope-labeled fumaric acid and NMR structural analysis method of protein

机译：内置有稳定同位素标记氨基酸的目标蛋白质的方法，酒石酸和区域选择性稳定同位素标记富马酸的生产方法以及蛋白质的NMR结构分析方法

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摘要

A least one of the amino acids constituting the protein, the following labeling patterns; Hydrogen of the methyl group and the other completely is deuterated other leaving a hydrogen of at least one of the methylene hydrogens in the methylene group or two or more (a) 1 In the group gem- a methyl (b) prochiral the deuterated other leaving one hydrogen, the hydrogen of the methyl group are partially deuterated are deuterated in (c) prochiral methyl groups (d) methyl group moiety aromatic ring hydrogenation To provide a stable isotope-labeled amino acids, characterized in that it has at least one of which is deuterated. By using this stable isotope-labeled amino acids, rapid and reliable analysis of the NMR spectrum of high molecular weight proteins to achieve without damaging the NMR sensitivity of the residual hydrogen nucleus, beyond the limits of conventional, deuterium substitution of the protein, It is possible to simultaneously achieve high-precision determination of the three-dimensional structure.

机译：构成蛋白质的至少一种氨基酸，具有以下标记方式;甲基氢和另一个氢完全氘化，剩下至少一个亚甲基氢中的一个或两个或多个亚甲基氢的氢（a）1在基团-甲基（b）中，氘代另一个手性（c）前手性甲基（d）甲基部分的芳香环氢化是氢的甲基，部分氢是氘代的。为了提供稳定的同位素标记的氨基酸，其特征在于它具有至少一个氘化。通过使用这种稳定的同位素标记的氨基酸，可以对高分子量蛋白质的NMR光谱进行快速而可靠的分析，而不会损害残留氢核的NMR灵敏度，这超出了蛋白质的常规氘取代的限制，这是可以同时实现三维结构的高精度确定。

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公开/公告号JPWO2003053910A1

专利类型
公开/公告日2005-04-28

原文格式PDF
申请/专利权人独立行政法人科学技術振興機構;
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申请/专利号JP20030554627
发明设计人甲斐荘正恒;寺内勉;
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申请日2002-12-19
分类号C07C229/08;C07C51/09;C07C57/15;C07C59/255;C07C229/22;C07C229/24;C07C229/36;C07C323/58;C07K1/13;C12P21/02;
国家 JP
入库时间 2022-08-21 22:26:39

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