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首页> 外文期刊>Journal of tissue engineering and regenerative medicine >Tissue responses against tissue-engineered cartilage consisting of chondrocytes encapsulated within non-absorbable hydrogel
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Tissue responses against tissue-engineered cartilage consisting of chondrocytes encapsulated within non-absorbable hydrogel

机译：针对由包裹在不可吸收水凝胶中的软骨细胞组成的组织工程软骨的组织反应

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摘要

To disclose the influence of foreign body responses raised against a non-absorbable hydrogel consisting of tissue-engineered cartilage, we embedded human/canine chondrocytes within agarose and transplanted them into subcutaneous pockets in nude mice and donor beagles. One month after transplantation, cartilage formation was observed in the experiments using human chondrocytes in nude mice. No significant invasion of blood cells was noted in the areas where the cartilage was newly formed. Around the tissue-engineered cartilage, agarose fragments, a dense fibrous connective tissue and many macrophages were observed. On the other hand, no cartilage tissue was detected in the autologous transplantation of canine chondrocytes. Few surviving chondrocytes were observed in the agarose and no accumulation of blood cells was observed in the inner parts of the transplants. Localizations of IgG and complements were noted in areas of agarose, and also in the devitalized cells embedded within the agarose. Even if we had inhibited the proximity of the blood cells to the transplanted cells, the survival of the cells could not be secured. We suggest that these cytotoxic mechanisms seem to be associated not only with macrophages but also with soluble factors, including antibodies and complements.
机译：为了揭示异物反应对由组织工程软骨组成的不可吸收水凝胶的影响，我们将人/犬软骨细胞嵌入琼脂糖中，并将其移植到裸鼠和供体小猎犬的皮下口袋中。移植后一个月，在裸鼠中使用人软骨细胞的实验中观察到软骨形成。在新形成软骨的区域未观察到明显的血细胞浸润。在组织工程的软骨周围，观察到了琼脂糖碎片，致密的纤维结缔组织和许多巨噬细胞。另一方面，在犬软骨细胞的自体移植中未检测到软骨组织。在琼脂糖中几乎观察不到存活的软骨细胞，在移植物的内部未观察到血细胞的积累。 IgG和补体的定位在琼脂糖区域中以及在琼脂糖内包埋的失活细胞中被注意到。即使我们抑制了血细胞与移植细胞的接近，也无法确保细胞的存活。我们建议这些细胞毒性机制似乎不仅与巨噬细胞有关，而且与可溶性因子（包括抗体和补体）有关。

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