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首页> 外文期刊>Journal of Veterinary Diagnostic Investigation >Measurement of urinary F2-isoprostanes as markers of in vivo lipid peroxidation: a comparison of enzyme immunoassays with gas chromatography-mass spectrometry in domestic animal species.
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Measurement of urinary F2-isoprostanes as markers of in vivo lipid peroxidation: a comparison of enzyme immunoassays with gas chromatography-mass spectrometry in domestic animal species.

机译：尿中F2-异前列腺素的测定，作为体内脂质过氧化的标志物：家畜中酶联免疫吸附测定与气相色谱-质谱联用的比较。

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摘要

F(2)-isoprostanes are useful markers for assessing oxidant injury; however, the validity of measuring urinary 15-F(2t)-isoprostane concentration by enzyme-linked immunosorbent assay (ELISA) has not been evaluated in veterinary species. The current study assesses the agreement between 2 commercially available urinary isoprostane kits and gas chromatography and negative ion chemical ionization-mass spectrometry (GC/NICI-MS). The results indicate that only feline urinary isoprostane measurement by glucuronidase (GL)-ELISA has acceptable agreement with GC/NICI-MS. Urinary isoprostane concentration was highly variable in critically ill animals, but there were too many variations between healthy and critically ill animals to draw meaningful conclusions. Currently, GC/NICI-MS is the only method that can be recommended for the assessment of urinary isoprostanes in dogs, cattle, and horses. Feline urinary isoprostanes can be assessed by GL-ELISA, but caution is still warranted when comparing data from manuscripts using different methods given the relatively low Spearman rank correlation coefficient. Future studies may require large sample sizes or focused inclusion criteria to account for variability in isoprostane concentration.
机译：F（2）-异前列腺素是评估氧化剂损伤的有用标记物;但是，尚未通过兽医学方法评估通过酶联免疫吸附法（ELISA）测定尿中15-F（2t）-异前列腺素浓度的有效性。本研究评估了2种市售尿异前列腺素试剂盒和气相色谱仪以及负离子化学电离质谱法（GC / NICI-MS）之间的协议。结果表明，仅通过葡萄糖醛酸苷酶（GL）-ELISA测定猫尿中尿前列腺素与GC / NICI-MS可以接受。危重动物的尿中异前列腺素浓度变化很大，但是健康和危重动物之间的尿酸差异太大，无法得出有意义的结论。当前，GC / NICI-MS是可推荐用于评估狗，牛和马中尿异前列腺素的唯一方法。猫尿中的异前列腺素可以通过GL-ELISA进行评估，但是在使用不同方法比较来自手稿的数据时，仍然需要谨慎，因为Spearman等级相关系数相对较低。未来的研究可能需要大样本量或集中的纳入标准，以说明异前列腺素浓度的变化。

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