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首页> 外文期刊>Chemical research in toxicology >Quantification of Urinary Excretion of 1,N~6-Ethenoadenine,a Potential Biomarker of Lipid Peroxidation,in Humans by Stable Isotope Dilution iquid Chromatography-Electrospray onization-Tandem Mass Spectrometry:Comparison ith Gas Chromatography-Mass Sp
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Quantification of Urinary Excretion of 1,N~6-Ethenoadenine,a Potential Biomarker of Lipid Peroxidation,in Humans by Stable Isotope Dilution iquid Chromatography-Electrospray onization-Tandem Mass Spectrometry:Comparison ith Gas Chromatography-Mass Sp

机译：稳定同位素稀释-液相色谱-电喷雾质谱-串联质谱法定量分析人体中脂质过氧化的潜在生物标志物1，N〜6-乙腺嘌呤的尿排泄量：气相色谱-质谱法比较

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Etheno DNA adducts are promutagenic DNA lesions derived from exogenous as well as endogenous sources.The levels of etheno adducts in tissue DNA are elevated in cancer prone tissues,and the urinary excretion of etheno adducts is associated with oxidative stress.In this report,a new assay based on isotope dilution liquid Chromatography-electrospray ionization-tandem mass Spectrometry (LC-ESI-MS/MS) is developed for the quantification of 1,N~6-ethenoadenine (epsilonAde) in human urine samples without the need for derivatization.Sample purification before analysis by MS only requires a reversed phase solid phase extraction column.Two multiple reaction monitoring transitions with two product ion fragments generated from a common parent ion were used to quantify urinary epsilonAde.The detection limit of epsilonAde using LC-ESI-MS/MS is 2 pg injected standard eAde on-column,and the assay allows accurate quantification of urinary eAde at concentrations higher than 10 pg/mL.The presence of eAde in human urine is confirmed by the collision-induced daughter ion spectrum.Using this assay,the levels of eAde in the 24 h urine samples from 18 healthy individuals are determined,and the results are in very good agreement with those obtained using isotope dilution gas chromatography-negative ion chemical ionization-mass Spectrometry.The high specificity and simple sample pretreatment of this LC-ESI-MS/MS method render it a valuable tool in measuring eAde in the complex mixture of human urine as a promising noninvasive biomarker for DNA damage associated with oxidative stress and for cancer chemoprevention studies.
机译：乙脑DNA加合物是外源性和内源性来源的致突变性DNA损伤。组织DNA中乙炔加合物的水平在易癌组织中升高，并且乙炔加合物的尿排泄与氧化应激有关。在本报告中，一种新的建立了基于同位素稀释液相色谱-电喷雾电离串联质谱（LC-ESI-MS / MS）的检测方法，无需进行衍生化即可对人尿液样品中的1，N〜6-乙炔腺嘌呤（epsilonAde）进行定量。质谱分析前的纯化仅需使用反相固相萃取柱即可进行。两个监测反应的过渡均由一个共同的母体离子产生的两个产物离子片段用于定量尿中ε酰胺.LC-ESI-MS / MS是柱上注射的2 pg标准eAde，该测定法可对浓度高于10 pg / mL的尿中eAde进行准确定量。通过碰撞诱导的子离子光谱确认人尿中的尿样。使用该测定法，测定了18个健康个体的24 h尿液样本中的eAde水平，其结果与使用同位素稀释气获得的结果非常吻合液相色谱-负离子化学电离质谱法。这种LC-ESI-MS / MS方法的高特异性和简单的样品预处理使其成为测量人类尿液复杂混合物中eAde的有价值的工具，作为有希望的DNA损伤无创生物标记物与氧化应激有关，并用于癌症化学预防研究。

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