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首页> 外文期刊>Journal of Veterinary Diagnostic Investigation >Diagnostic accuracy of methods for detecting Anaplasma marginale infection in lactating dairy cattle of Puerto Rico.
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Diagnostic accuracy of methods for detecting Anaplasma marginale infection in lactating dairy cattle of Puerto Rico.

机译：波多黎各泌乳奶牛边缘Ana质感染检测方法的诊断准确性。

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Bovine anaplasmosis (BA) is a hemoparasitic disease of great importance in cattle within the tropical and subtropical regions of the world. Control programs for BA require accurate diagnostic assays but validation can be challenging because the true disease status of all animals is frequently not known with certainty. The objective of this study was to estimate the accuracy of assays for detection of Anaplasma marginale infection in lactating dairy cattle of Puerto Rico using Bayesian methods without a perfect reference test. There were 2,331 cattle with complete diagnostic results sampled from 79 herds, and the prevalence of BA was estimated as 22% (95% probability interval [PI]: 19-25%). The sensitivity (Se) and specificity (Sp) of a major surface protein 5 competitive enzyme-linked immunosorbent assay (MSP-5 cELISA) were estimated as 99% (95% PI: 96-100%) and 89% (95% PI: 87-92%), respectively. The Se and Sp of a quantitative polymerase chain reaction (qPCR) were 67% (95% PI: 60-74%) and 99% (95% PI: 99-100%). The Se and Sp of a card agglutination test were 34% (95% PI: 29-39%) and 99% (95% PI: 99-100%). Area under the receiver-operating characteristic curve for the MSP-5 cELISA was 0.748 (95% PI: 0.71-0.79). The MSP-5 cELISA appears to be the test of choice for screening cattle for subclinical BA based on the high estimated Se, rapidity of results, relative low cost, and ease of standardization.
机译：在世界热带和亚热带地区的牛中，牛无性病（BA）是一种非常重要的血液寄生虫病。 BA的控制程序需要准确的诊断检测方法，但由于常常无法确定所有动物的真实疾病状况，因此验证可能具有挑战性。这项研究的目的是评估使用贝叶斯方法在没有完善参考测试的情况下检测波多黎各泌乳奶牛边缘无浆膜感染的检测方法的准确性。从79个牛群中采集了2331头牛，具有完整的诊断结果，估计BA的患病率为22％（95％概率区间[PI]：19-25％）。主要表面蛋白5竞争性酶联免疫吸附测定（MSP-5 cELISA）的灵敏度（Se）和特异性（Sp）估计为99％（95％PI：96-100％）和89％（95％PI） ：87-92％）。定量聚合酶链反应（qPCR）的Se和Sp分别为67％（95％PI：60-74％）和99％（95％PI：99-100％）。卡凝集测试的Se和Sp分别为34％（95％PI：29-39％）和99％（95％PI：99-100％）。 MSP-5 cELISA的受体工作特征曲线下的面积为0.748（95％PI：0.71-0.79）。 MSP-5 cELISA似乎是根据高估计的Se含量，快速的结果，相对较低的成本以及易于标准化的方法筛查牛亚临床BA的首选测试方法。

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