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首页> 外文期刊>Journal of Veterinary Research >Immunophenotypic characterisation and cytogenetic analysis of mesenchymal stem cells from equine bone marrow and foal umbilical cords during in vitro culture
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Immunophenotypic characterisation and cytogenetic analysis of mesenchymal stem cells from equine bone marrow and foal umbilical cords during in vitro culture

机译：马骨髓和小马脐带间充质干细胞体外培养的免疫表型鉴定和细胞遗传学分析

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Introduction: The objective of the study was immunophenotypic and cytogenetic analysis of mesenchymal stem cells from equine bone marrow and foal umbilical cords during in vitro culture. Material and Methods: The mesenchymal stem cells were obtained from equine bone marrow of three horses and from foal umbilical cords of six foals. The cells were cultured in CO2 incubators by standard procedure. Quantitative abnormalities of chromosomes, i.e. aneuploidy and polyploidy, and structural aberrations, i,e. breaks in chromosomes and chromatid, were taken into account during the study. Results: The results of cytogenetic analysis of equine bone marrow mesenchymal stem cells at the third and fourth passages indicated that the level of karyotype variability of these cells corresponded to the spontaneous level of karyotype variability typical of the peripheral blood lymphocytes of this species. Equine bone marrow contained several clones of stem cells that differed in the expression of specific nuclear markers characteristic of proliferating cells. Conclusion: Mesenchymal stem cells from foal umbilical cords during in vitro cultivation are characterised by quantitative abnormalities of the chromosomal apparatus.
机译：简介：该研究的目的是在体外培养过程中对马骨髓和小马脐带间充质干细胞进行免疫表型和细胞遗传学分析。材料与方法：间充质干细胞取自三匹马的马骨髓和六只驹的驹脐带。通过标准程序在CO2培养箱中培养细胞。染色体的定量异常，即非整倍性和多倍体，以及结构畸变，即在研究过程中考虑了染色体和染色单体的断裂。结果：第三和第四代马骨髓间充质干细胞的细胞遗传学分析结果表明，这些细胞的核型变异性水平与该物种外周血淋巴细胞典型的核型变异性自然水平相对应。马骨髓含有几个干细胞克隆，这些克隆在增殖细胞特有的特定核标记物的表达方面有所不同。结论：体外培养的小马驹脐带间充质干细胞具有染色体仪器定量异常的特征。

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