...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of vascular research >Expression of membrane-type matrix metalloproteinase in rabbit neointimal tissue and its correlation with matrix-metalloproteinase-2 activation.
【24h】

Expression of membrane-type matrix metalloproteinase in rabbit neointimal tissue and its correlation with matrix-metalloproteinase-2 activation.

机译：膜型基质金属蛋白酶在兔新生内膜组织中的表达及其与基质金属蛋白酶2活化的关系。

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Smooth muscle cell (SMC) phenotypic alteration, followed by migration and proliferation, is a prominent feature of atherogenesis and vascular neointimal formation. Despite extensive research, mechanism(s) responsible for this alteration remain unclear. Recently, matrix metalloproteinases (MMP), a family of potent proteinases, have been implicated in vascular diseases by way of extracellular matrix degradation. Of particular interest is that expression of a 72-kD MMP (MMP-2) is elevated in neointima, and inhibition of this MMP results in reduced SMC migration and proliferation, suggesting a role for MMP-2 in neointimal development. However, MMP-2 needs activation before digesting protein; the mechanism of this activation in the arterial wall is largely unexplored. A novel membrane-type MMP termed MT-MMP-1 has recently been identified, and its expression in tumor cells is concomitant with MMP-2 activation. Transfection of this MMP cDNA into mammalian cells results in activation of MMP-2. However, the importance of this MMP in various pathological situations is not clear. The present study was designed to explore the relationship between MT-MMP- 1 expression and MMP-2 activation during rabbit neointimal development. Using polymerase chain reaction, we isolated a rabbit cDNA from arterial SMC; sequence analysis indicated that it is a rabbit form ofMT-MMP-1. A segment of this cDNA was subcloned into pGEM-3 and employed to synthesize a DIG-labeled RNA probe. This probe was then used in the Northern blot analysis for MT-MMP-1 mRNA expression both in aortic tissue and in neointimal tissues developed 3, 7, 14 and 21 days after balloon catheter de-endothelialization. The results show low-level expression ofMT-MMP-1 in the normal aortic wall; expression is significantly increased in the neointimal tissues, with peak expression observed in tissues 3 days after injury. Expression of active MMP-2 was also determined using gel zymography. A close temporal expression pattern was observed between MT-MMP-1 and active MMP-2. These data verify the expression of MT-MMP-1 in arterial SMC and suggest its importance in MMP-2 activation after balloon catheter de-endothelialization.
机译：平滑肌细胞（SMC）的表型改变，然后迁移和增殖，是动脉粥样硬化和血管新生内膜形成的突出特征。尽管进行了广泛的研究，但仍不清楚导致这种改变的机制。近来，基质金属蛋白酶（MMP），一种有效的蛋白酶家族，已通过细胞外基质降解与血管疾病有关。特别令人感兴趣的是在新内膜中72kD MMP（MMP-2）的表达升高，并且对该MMP的抑制导致SMC迁移和增殖减少，提示MMP-2在新内膜发育中的作用。但是，MMP-2在消化蛋白质之前需要激活。这种激活在动脉壁的机制在很大程度上尚待探索。最近已鉴定出一种新型膜型MMP，称为MT-MMP-1，其在肿瘤细胞中的表达与MMP-2的激活同时发生。将该MMP cDNA转染到哺乳动物细胞中会激活MMP-2。但是，这种MMP在各种病理情况下的重要性尚不清楚。本研究旨在探讨兔新内膜发育过程中MT-MMP-1表达与MMP-2激活之间的关系。使用聚合酶链反应，我们从动脉SMC分离了兔cDNA。序列分析表明它是MT-MMP-1的兔形式。将该cDNA的片段亚克隆到pGEM-3中，并用于合成DIG标记的RNA探针。然后将该探针用于Northern印迹分析中，在球囊导管去内皮化后3、7、14和21天，主动脉组织和新内膜组织中的MT-MMP-1 mRNA表达。结果表明MT-MMP-1在正常主动脉壁中低水平表达;新生内膜组织中的表达显着增加，在损伤后3天在组织中观察到峰值表达。活性MMP-2的表达也使用凝胶酶谱法测定。在MT-MMP-1和活跃的MMP-2之间观察到了紧密的时间表达模式。这些数据验证了MT-MMP-1在动脉SMC中的表达，并暗示了其在球囊导管去内皮化后在MMP-2激活中的重要性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号