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ACTIVITY OF NATURAL PRODUCTS AGAINST SOME PHYTOPATHOGENIC FUNGI

机译:天然产物对某些植物病原真菌的活性

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The requirement of environmental protection and food safety is perceived with always major interest by public opinion and it is consistent with European Union legislation on the sustainable use of pesticides (Directive 2009/128/EC). This directive requires member states to promote low pesticide-input, giving priority to non-chemical methods and low risk plant protection products. In order to contribute to the achievement of these objectives antifungal activity of natural substances, characterized bya good toxico-logical and ecotoxicological profile, was tested. Essential oil of Melaleuca alternifolia, essential oil of Syzygium aromaticum and extract from Mimosa tenuiflora were tested against Alternaria alternata, Bo-trytis cinerea and Fusarium oxysporum f. sp. lycopersici (races 1 and 2). In vitro tests involved determination of radial growth of the colonies of fungi in the presence of varying concentrations of tested products in agar media and determination of germination percentage in the presence of tested product at various concentrations. The products based on essential oil of M. alternifolia were also tested in vivo on tomato fruits wounded and artificially inoculated with A. alternata or with B. cinerea. The in vitro tests showed the antifungal activity of both essential oils instead the extract from M. tenuiflora exhibited poor antifungal activity and only against A. alternata and B. cinerea. The results on tomato fruits showed inhibition of grey mould and black mould by essential oil ofM. alternifolia. The antifungal activity increased with increasing concentrations. In conclusion, the obtained results in the present study showed promising prospects for the utilisation of investigated products to reduce the using of antifungal chemicals and to achieve a more sustainable use of pesticides.
机译:公众一直对环境保护和食品安全的要求始终抱有极大的兴趣,这与欧盟关于农药可持续使用的法规(指令2009/128 / EC)保持一致。该指令要求成员国提倡低农药投入,优先采用非化学方法和低风险的植物保护产品。为了有助于实现这些目标,测试了以良好的毒理学和生态毒理学特征为特征的天然物质的抗真菌活性。测试了互叶白千层精油,香蒲和精制含羞草的提取物对链格孢,灰霉菌和尖孢镰刀菌f的影响。 sp。 lycopersici(竞赛1和2)。体外试验涉及在琼脂培养基中存在变化浓度的被测产物的情况下确定真菌菌落的径向生长,以及在不同浓度的被测产物的存在下确定发芽率。还基于伤寒假单胞菌或灰葡萄孢的人工接种的番茄果实,在体内测试了基于链格孢菌的精油的产品。体外试验表明,两种精油均具有抗真菌活性,而ten.flora的提取物则显示出较弱的抗真菌活性,并且仅对互生曲霉和灰质芽孢杆菌具有抗真菌活性。番茄果实的结果表明,M精油对灰霉病和黑霉病具有抑制作用。互生叶。抗真菌活性随浓度增加而增加。总而言之,本研究获得的结果显示了利用被调查产品减少抗真菌化学品的使用并实现更可持续使用农药的前景广阔的前景。

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