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首页> 外文期刊>Journal of Virological Methods >Prospective comparison of Abbott RealTime HBV DNA and Versant HBV DNA 3.0 assays for hepatitis B DNA quantitation: Impact on HBV genotype monitoring
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Prospective comparison of Abbott RealTime HBV DNA and Versant HBV DNA 3.0 assays for hepatitis B DNA quantitation: Impact on HBV genotype monitoring

机译：雅培RealTime HBV DNA和Versant HBV DNA 3.0测定法对乙肝DNA定量的前瞻性比较：对HBV基因型监测的影响

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摘要

The quantitation of human hepatitis B virus (HBV) in the serum of infected patients is recommended to characterize the course of chronic HBV infection. The aim of this prospective study was to evaluate the performance of the Abbott RealTime PCR assay for HBV DNA quantitation by comparison with the standard Versant HBV DNA 3.0 assay. The better sensitivity and broader dynamic range of HBV DNA quantitation using the Abbott RealTime PCR assay was confirmed by the study of 362 serum samples from 311 patients. In addition, data analysis revealed the concordance of HBV DNA quantitations between the two assays. When this evaluation was assessed as a function of HBV genotype, there was discordance for HBV genotype C samples. Thus, we performed an in-house PCR to confirm the discrepancy observed regarding the HBV genotypes. The in-house PCR results agreed better with the Abbott RealTime PCR method when compared with the standard hybridization assay. In conclusion, the wide dynamic range of HBV DNA quantitation achieved with the Abbott RealTime PCR assay makes it appropriate for the clinical monitoring of HBV infected patients. However, a change of HBV DNA quantitation method could influence results on the follow-up of HBV genotype C infected patients.
机译：建议对感染患者血清中的人类乙型肝炎病毒（HBV）进行定量，以表征慢性HBV感染的过程。这项前瞻性研究的目的是通过与标准Versant HBV DNA 3.0分析法进行比较，评估Abbott RealTime PCR分析法对HBV DNA定量的性能。通过对311位患者的362个血清样本进行的研究，证实了使用Abbott RealTime PCR检测法对HBV DNA定量分析具有更好的灵敏度和更宽的动态范围。另外，数据分析显示两种测定之间HBV DNA定量的一致性。当评估此评估为HBV基因型的函数时，对于HBV基因型C样本存在不一致。因此，我们进行了室内PCR来确认观察到的有关HBV基因型的差异。与标准杂交法相比，内部PCR结果与Abbott RealTime PCR方法具有更好的一致性。总之，通过雅培实时荧光定量PCR检测可实现广泛的HBV DNA定量动态范围，因此非常适合对HBV感染患者进行临床监测。但是，改变HBV DNA定量方法可能会影响对HBV基因型C型感染患者的随访结果。

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