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首页> 外文期刊>Journal of Wildlife Management >Effects of two porcine zona pellucida immunocontraceptive vaccines on ovarian activity in horses.
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Effects of two porcine zona pellucida immunocontraceptive vaccines on ovarian activity in horses.

机译：两种猪透明带免疫避孕疫苗对马卵巢活性的影响。

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Feral horse population growth rates as high as 25% are of concern to those responsible for managing range lands as well as conservation groups. Current methods to control these populations include adoption and long-term holding, which are both costly and controversial. Porcine zona pellucida (pZP) immunocontraception may have the greatest potential to control fertility because it has proven to be effective in other studies and vaccines are easy and safe to administer. One pZP vaccine formulation, SpayVacReg. (ImmunoVaccine Technologies, Inc., Halifax, NS, Canada), has demonstrated single-dose, multi-year contraceptive efficacy in other wildlife species, which would make it both practical and economical for field application. Over a 7-month period during the breeding season, we assessed the effect on ovarian activity of 2 formulations of SpayVac, 1 non-aqueous with modified Freund's adjuvant (MFA) and the other, an aqueous emulsion with MFA, compared to controls (n=7 per group). Comparative reproductive parameters included serum concentrations of progesterone (P4) determined by enzyme-linked immunosorbent assay (ELISA), ovarian activity assessed by transrectal ultrasound and palpation, as well as gross and histological examination of ovaries upon necropsy (n=9 or 3 mares from each group) or after ovariectomy (n=12 or 4 mares from each group). We determined serum antibody titers using ELISA. Mean serum concentrations of P4 were less in the non-aqueous MFA treatment group compared to control mares (P<0.025). Ovaries collected from control mares weighed more (P=0.002) and had greater variation (P=0.003) than those from either vaccinated group. Both treatment groups also had smaller ovaries and fewer follicles compared to controls (P<0.001). Three to 4 months after vaccination, 93% of SpayVac-injected mares ceased cycling; whereas all control mares continued to cycle throughout the study. Relatively constant antibody titers were reached by week 6 post-vaccination, although we found appreciable variation within treatment groups, especially 4-8 weeks post-vaccination. Based on our study, the SpayVac formulations impair ovarian function but do not affect other major organ systems, and could provide a safe and effective immunocontraceptive option for mares. Additional research to elucidate the vaccine's mechanism of action, actual contraceptive efficacy, and long-term effects are still needed.
机译：负责管理牧场和保护区的人们关注着高达25％的野生马人口增长率。当前控制这些人群的方法包括收养和长期持有，这既昂贵又有争议。猪透明带（pZP）免疫避孕可能具有最大的控制生育力的潜力，因为它已在其他研究中证明是有效的，并且疫苗易于安全接种。一种pZP疫苗制剂SpayVacReg。 （ImmunoVaccine Technologies，Inc.，加拿大新罕布什尔州哈利法克斯）在其他野生动植物物种中已证明了单剂量，多年的避孕功效，这使其在现场应用中既实用又经济。在繁殖季节的7个月内，我们评估了2种SpayVac制剂对卵巢活性的影响，其中1种非水性改良弗氏佐剂（MFA）和另一种与MFA混合的水性乳剂与对照组相比（n =每组7个）。比较生殖参数包括通过酶联免疫吸附测定（ELISA）测定的孕酮血清浓度（P 4 ），经直肠超声和触诊评估的卵巢活性以及尸检后卵巢的总体和组织学检查（每组n = 9或3匹母马）或卵巢切除术后（每组n = 12或4匹母马）。我们使用ELISA测定了血清抗体滴度。非水MFA治疗组的平均血清P 4 浓度低于对照组母马（P <0.025）。与任一疫苗接种组相比，从对照母马收集的卵巢重量更大（P = 0.002），变异性更大（P = 0.003）。与对照组相比，两个治疗组的卵巢都更小，卵泡也更少（P <0.001）。接种疫苗三到四个月后，注射SpayVac的母马中有93％停止了骑行;而所有对照母马在整个研究过程中继续循环。疫苗接种后第6周达到相对恒定的抗体滴度，尽管我们发现治疗组之间存在明显差异，尤其是疫苗接种后4-8周。根据我们的研究，SpayVac制剂损害卵巢功能，但不影响其他主要器官系统，并且可以为母马提供安全有效的免疫避孕选择。仍需要进一步研究以阐明疫苗的作用机理，实际避孕功效和长期效果。

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