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首页> 外文期刊>Journal of orofacial pain. >Effects of Low-Intensity Pulsed Ultrasound on the Expression of Cyclooxygenase-2 in Mandibular Condylar Chondrocytes
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Effects of Low-Intensity Pulsed Ultrasound on the Expression of Cyclooxygenase-2 in Mandibular Condylar Chondrocytes

机译：低强度脉冲超声对下颌Con突软骨细胞环氧合酶-2表达的影响

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Aims: To determine the effect of low-intensity pulsed ultrasound (LIPUS) on cyclooxygenase-2 (COX-2) expression and related mechanisms by using cultured articular chondrocytes derived from porcine mandibular condyles after treatment with interleukin-1 beta(IL-1beta). Methods: Chondrocytes were derived from porcine mandibular condylar cartilage and cultured. The cells were treated with or without 10 ng/mL IL-1beta. At the same time, the cells were exposed to LIPUS for 20 minutes. After LIPUS exposure, the conditioned medium was changed to a fresh one without IL-1beta, and the cells were incubated for 0 to 24 hours. The effects of LIPUS on IL-1beta-treated chondrocytes were examined in terms of the expression of p-integrin beta1, COX-2, and phosphorylated extracellular signal-related kinase (p-ERK) 1/2 by real-time polymerase chain reaction (PCR) and Western blot analyses. Differences in the means among multiple groups were examined by oneway analysis of variance (AN0VA) for all groups at each time point, followed by a Scheffe multiple comparison test as a post-hoc test; Student t test was also used. Results: COX-2 mRNA level was upregulated by the treatment with IL-1beta and was suppressed significantly (P < .01) by LIPUS exposure. Furthermore, LIPUS enhanced gene expression and phosphorylation of integrin beta, and it inhibited the expression of p-ERK 1/2. Conclusion: LIPUS exposure inhibited IL-1beta-induced COX-2 expression through the integrin beta1 receptor followed by the phosphorylation of ERK 1/2. Despite the restricted duration of its effect, LIPUS is suggested to be a potential candidate as a preventive and auxiliary treatment to suppress the degradation of articular chondrocytes in temporomandibular joint osteoarthritis.
机译：目的：通过白介素1β（IL-1β）处理后的猪下颌con突关节软骨细胞，研究低强度脉冲超声（LIPUS）对环氧合酶2（COX-2）表达的影响及其相关机制。方法：软骨细胞取自猪下颌con突软骨并进行培养。用或不用10 ng / mL IL-1beta处理细胞。同时，将细胞暴露于LIPUS 20分钟。 LIPUS暴露后，将条件培养基更换为不含IL-1beta的新鲜培养基，并将细胞孵育0至24小时。通过实时聚合酶链反应，通过对p-1整合素β1，COX-2和磷酸化的细胞外信号相关激酶（p-ERK）1/2的表达，检查了LIPUS对IL-1beta处理的软骨细胞的影响。 （PCR）和Western印迹分析。通过在每个时间点对所有组的单因素方差分析（AN0VA）检验多个组之间均值的差异，然后进行Scheffe多重比较检验作为事后检验;还使用了学生t检验。结果：IL-1β处理可上调COX-2 mRNA的水平，LIPUS暴露可显着抑制COX-2 mRNA的水平（P <.01）。此外，LIPUS增强了整联蛋白β的基因表达和磷酸化，并抑制了p-ERK 1/2的表达。结论：LIPUS暴露通过整联蛋白beta1受体抑制了IL-1beta诱导的COX-2表达，随后使ERK 1/2磷酸化。尽管其作用的持续时间有限，但仍建议将LIPUS用作预防和辅助治疗以抑制颞下颌关节骨关节炎中关节软骨细胞降解的潜在候选药物。

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