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首页> 外文期刊>Journal of cellular biochemistry. >Sox9 transactivation and testicular expression of a novel human gene, KIAA0800.
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Sox9 transactivation and testicular expression of a novel human gene, KIAA0800.

机译：一个新的人类基因KIAA0800的Sox9反式激活和睾丸表达。

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摘要

The Sry and Sox9 sex-determination factors initiate and promote testis differentiation by gene transactivation through similar promoter elements. However, knowledge is limited concerning what genes are regulated by Sry/Sox9 in the testis. Identification and characterization of Sry/Sox9-regulated genes are critical for understanding sexual differentiation. We now demonstrate that a novel human gene, KIAA0800, is preferentially expressed in the testis and is transactivated by Sox9. The KIAA0800 promoter is repressed by an upstream element involving a polyT track and two Alu repeats. Two specific Sox9-bindings sites have been identified in the KIAA0800 promoter by using DNaseI footprinting assays and gel electrophoretic mobility shift assays. Sox9 transactivation of the KIAA0800 promoter appears to be exerted mainly through the relief of promoter repression. Genes homologous to the human KIAA0800 exist in organisms with differentiated sex tissues including mouse, Drosophila, and C. elegans, but not in unicellular organisms, including yeast and bacteria. Further, our recent sequence analysis shows that KIAA0800 protein is 97% identical between human and mouse. Thus, KIAA0800 is a novel Sox9-activated gene that is evolutionarily conserved and potentially involved in sexual differentiation.
机译：Sry和Sox9性别决定因子通过类似启动子元件的基因反式激活来启动和促进睾丸分化。但是，关于睾丸中Sry / Sox9调控哪些基因的知识有限。 Sry / Sox9调控基因的鉴定和表征对于理解性别分化至关重要。现在，我们证明一种新的人类基因KIAA0800在睾丸中优先表达并被Sox9反式激活。通过涉及polyT轨道和两个Alu重复序列的上游元件抑制KIAA0800启动子。通过使用DNaseI足迹分析和凝胶电泳迁移率迁移分析，已在KIAA0800启动子中鉴定出两个特定的Sox9结合位点。 KIAA0800启动子的Sox9反式激活似乎主要是通过缓解启动子阻抑来实现的。与人KIAA0800同源的基因存在于具有分化性组织的生物中，包括小鼠，果蝇和秀丽隐杆线虫，但在单细胞生物中不存在，包括酵母和细菌。此外，我们最近的序列分析表明，KIAA0800蛋白在人和小鼠之间的同源性为97％。因此，KIAA0800是一个新的Sox9激活基因，在进化上是保守的，并可能参与性别分化。

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