...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of cellular biochemistry. >The Phytoestrogen Genistein Affects Breast Cancer Cells Treatment Depending on the ER alpha/ER beta Ratio
【24h】

The Phytoestrogen Genistein Affects Breast Cancer Cells Treatment Depending on the ER alpha/ER beta Ratio

机译：植物雌激素染料木黄酮影响乳腺癌细胞的治疗，取决于ER alpha / ER beta比率

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Genistein (GEN) is a phytoestrogen found in soybeans. GEN exerts its functions through its interaction with the estrogen receptors (ER), ER alpha and ER beta, and we previously reported that the ER alpha/ER beta ratio is an important factor to consider in GEN-treated breast cancer cells. The aim of this study was to investigate the effects of GEN in breast cancer cells with different ER alpha/ER beta ratio: MCF-7 (high ratio), T47D (low ratio), and MCF-7 overexpressing ER beta (MCF7 + ER beta) treated with cisplatin (CDDP), paclitaxel (PTX) or tamoxifen (TAM). Cell viability, ROS production, autophagy, apoptosis, antioxidant enzymes protein levels, and cell cycle were analyzed. GEN treatment provoked an increase in cell viability in MCF-7 cells and in the antioxidant enzymes protein levels in combination with the cytotoxic agents, decreasing ROS production (CDDP + GEN and TAM + GEN) and autophagy (TAM + GEN) or apoptosis (CDDP + GEN and TAM + GEN). Moreover GEN treatment enhanced the cell cycle S phase entry in CDDP | GEN- and TAM | GEN- treated MCF-7 cells and, in the case of CDDP + GEN, increased the proportion of cells in the G2/M phase and decreased it in the subG(0)/G(1) phase. Otherwise, in the T47D and MCF7 + ER beta cells the combination of GEN with cytotoxic treatments did not cause significant changes in these parameters, even TAM + GEN- treated T47D cells showed less cell viability due to an increment in the autophagy. In conclusion, GEN consumption may be counterproductive in those patients receiving anticancer treatment with a high ER alpha/ER beta ratio diagnosed breast cancer and it could be harmless or even beneficial in those patients with a lower ER alpha/ER beta ratio breast cancer cells. (C) 2015 Wiley Periodicals, Inc.
机译：Genistein（GEN）是大豆中发现的一种植物雌激素。 GEN通过与雌激素受体（ER），ERα和ERβ相互作用来发挥其功能，我们之前曾报道ERα/ ERβ比是在GEN治疗的乳腺癌细胞中要考虑的重要因素。这项研究的目的是研究GEN在具有不同ER alpha / ERβ比值的乳腺癌细胞中的作用：MCF-7（高比值），T47D（低比值）和MCF-7过表达ERβ（MCF7 + ER） β）用顺铂（CDDP），紫杉醇（PTX）或他莫昔芬（TAM）处理。分析细胞活力，ROS产生，自噬，细胞凋亡，抗氧化酶蛋白水平和细胞周期。 GEN处理与细胞毒性剂联合使用可引起MCF-7细胞的细胞活力和抗氧化酶蛋白水平增加，从而降低ROS产生（CDDP + GEN和TAM + GEN）和自噬（TAM + GEN）或凋亡（CDDP） + GEN和TAM + GEN）。此外，GEN处理增强了CDDP中细胞周期S期的进入| GEN-和TAM | GEN处理的MCF-7细胞，在CDDP + GEN的情况下，增加了G2 / M期细胞的比例，降低了subG（0）/ G（1）期的细胞比例。否则，在T47D和MCF7 + ER beta细胞中，GEN与细胞毒性治疗的组合不会引起这些参数的显着变化，即使TAM + GEN处理的T47D细胞由于自噬的增加也显示出较低的细胞活力。总而言之，GEN消耗量在那些接受高诊断为ERα/ ERβ比值的乳腺癌患者接受抗癌治疗的患者中可能适得其反，并且对于那些具有较低ERα/ ERβ比值乳腺癌细胞的患者可能无害甚至有益。 （C）2015威利期刊公司

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号