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首页> 外文期刊>Journal of cellular biochemistry. >The roles of types XII and XIV collagen in fibrillogenesis and matrix assembly in the developing cornea.
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The roles of types XII and XIV collagen in fibrillogenesis and matrix assembly in the developing cornea.

机译：XII和XIV型胶原蛋白在角膜发育中的原纤维形成和基质组装中的作用。

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摘要

Corneal transparency depends on the architecture of the stromal extracellular matrix, including fibril diameter, packing, and lamellar organization. The roles of collagen types XII and XIV in regulation of corneal fibrillogenesis and development were examined. The temporal and spatial expression patterns were analyzed using semi-quantitative RT-PCR, in situ hybridization, Western analysis, and immunohistochemistry. Expression of types XII and XIV collagens in cornea development demonstrated that type XII collagen mRNA levels are constant throughout development (10D-adult) while type XIV mRNA is highest in early embryonic stages (10D-14D), decreasing significantly by hatching. The spatial expression patterns of types XII and XIV collagens demonstrated a homogeneous signal in the stroma for type XIV collagen, while type XII collagen shows segregation to the sub-epithelial and sub-endothelial stroma during embryonic stages. The type XII collagen in the anterior stroma was an epithelial product during development while fibroblasts contributed in the adult. Type XIV collagen expression was highest early in development and was absent by hatching. Both types XII and type XIV collagen have different isoforms generated by alternative splicing that may alter specific interactions important in fibrillogenesis, fibril-fibril interactions, and higher order matrix assembly. Analysis of these splice variants demonstrated that the long XII mRNA levels were constant throughout development, while the short XII NC3 mRNA levels peaked early (12D) followed by a decrease. Both type XIV collagen NC1 splice variants are highest during early stages (12D-14D) decreasing by 17D of development. These data suggest type XII collagen may have a role in development of stromal architecture and maintenance of fibril organization, while type XIV collagen may have a role in regulation of fibrillogenesis. J. Cell. Biochem. 87: 208-220, 2002. Copyright 2002 Wiley-Liss, Inc.
机译：角膜透明度取决于基质细胞外基质的结构，包括原纤维直径，堆积和层状组织。检查了XII和XIV型胶原在角膜原纤维形成和发育中的作用。使用半定量RT-PCR，原位杂交，Western分析和免疫组织化学分析了时空表达模式。 XII和XIV型胶原在角膜发育中的表达表明，XII型胶原mRNA的水平在整个发育过程中是恒定的（成人10D），而XIV型mRNA在早期胚胎阶段（10D-14D）最高，通过孵化显着降低。 XII和XIV型胶原的空间表达模式在XIV型胶原的基质中显示出均一的信号，而XII型胶原在胚胎期显示分离到上皮下和内皮下基质。前基质中的XII型胶原是发育过程中的上皮产物，而成纤维细胞在成体中起作用。 XIV型胶原蛋白的表达在发育早期最高，而孵化则不存在。 XII型胶原蛋白和XIV型胶原蛋白都具有通过替代剪接产生的不同同工型，它们可能会改变在原纤维形成，原纤维-原纤维相互作用和更高阶基质组装中重要的特定相互作用。对这些剪接变体的分析表明，长的XII mRNA水平在整个发育过程中是恒定的，而短的XII NC3 mRNA水平则在早期（12D）达到峰值，然后下降。 XIV型胶原蛋白NC1两种剪接变体在早期阶段（12D-14D）最高，而发育下降17D。这些数据表明XII型胶原蛋白可能在基质结构的发展和原纤维组织的维持中起作用，而XIV型胶原蛋白可能在原纤维形成的调节中起作用。 J.细胞。生化。 87：208-220，2002年。版权所有2002 Wiley-Liss，Inc.。

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