...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of cellular biochemistry. >Characterization of the nuclear matrix proteins in a transgenic mouse model for prostate cancer.
【24h】

Characterization of the nuclear matrix proteins in a transgenic mouse model for prostate cancer.

机译：前列腺癌转基因小鼠模型中核基质蛋白的表征。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The nuclear matrix (NM) contains a number of proteins that have been found to be associated with transformation. We have previously identified changes in the NM associated with prostate cancer. In this study, we examine the molecular changes that are associated with prostate cancer development in transgenic adenocarcinoma of mouse prostate (TRAMP) model by studying the differences in the NM proteins (NMPs). We collected prostates from the TRAMP males at six critical time points: 6 weeks (puberty), 11 and 19 weeks (development of mild hyperplasia), 25 weeks (development of severe hyperplasia), 31 and 37 weeks (development of neoplasia). The nuclear matrices from the prostates collected at these time points were then isolated and the NMPs were characterized by high-resolution two-dimensional gel electrophoresis. We found three NMPs (E1A, E1B, and E1C) that were present in the 6-week-old prostate and two NMPs (E2A and E2B) that were present in the 11-week-old prostate. These NMPs were absent in the 31- and 37-week-old prostate. We also found five NMPs (E3A-E3E) that were present in the 31-week-old prostate, but absent in the earlier time points. In addition, three NMPs (Le1, Le2, Le3) were present at higher expression in the 6-, 11-, 19-, and 25-weeks old TRAMP prostates, but they were expressed lower during the development of neoplasia at 31- and 37-weeks old. Identification of these NMPs permits the development of novel markers that can characterize various stages of prostate cancer development as well as potentially therapeutic targets.
机译：核基质（NM）包含许多与转化有关的蛋白质。我们先前已经确定了与前列腺癌相关的NM的变化。在这项研究中，我们通过研究NM蛋白（NMP）的差异，研究了与小鼠前列腺转基因腺癌​​（TRAMP）模型中的前列腺癌发展相关的分子变化。我们在六个关键时间点从TRAMP男性那里收集了前列腺：6周（青春期），11和19周（轻度增生的发展），25周（严重增生的发展），31和37周（瘤的发展）。然后，在这些时间点从前列腺中分离出核基质，并通过高分辨率二维凝胶电泳对NMP进行表征。我们发现存在于6周龄前列腺中的三个NMP（E1A，E1B和E1C）和存在于11周龄前列腺中的两个NMP（E2A和E2B）。这些NMP在31和37周龄的前列腺中不存在。我们还发现在31周龄的前列腺中存在五个NMP（E3A-E3E），但在较早的时间点不存在。此外，在6周，11周，19周和25周大的TRAMP前列腺中，三个NMP（Le1，Le2，Le3）以较高的表达存在，但在31岁和32岁的瘤形成过程中它们的表达较低。 37周大。这些NMPs的鉴定允许开发新的标志物，其可以表征前列腺癌发展的各个阶段以及潜在的治疗靶标。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号