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首页> 外文期刊>Biophysical Journal >Cholesterol interactions with fluid-phase phospholipids: effect on the lateral organization of the bilayer.
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Cholesterol interactions with fluid-phase phospholipids: effect on the lateral organization of the bilayer.

机译：胆固醇与液相磷脂的相互作用：对双层的横向组织的影响。

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摘要

The lateral organization of lipids and proteins in cell membranes is recognized as an important factor in several cellular processes. Cholesterol is thought to function as a modulator of the lateral segregation of lipids into cholesterol-poor and cholesterol-rich domains. We investigated how the affinity of cholesterol for different phospholipids, as seen in cholesterol partitioning between methyl-beta-cyclodextrin and large unilamellar vesicles, was reflected in the lateral organization of lipids in complex bilayers. We especially wanted to determine how the low-T(m) lipid affected the lateral structure. Partition experiments showed that cholesterol had a higher affinity for N-oleoyl-sphingomyelin (OSM) than for palmitoyl-oleoyl-phosphatidylcholine (POPC) bilayers, but the highest preference was for N-palmitoyl-sphingomyelin (PSM)-containing bilayers. Partial phase diagrams of POPC/PSM/cholesterol and OSM/PSM/cholesterol bilayers at 23 degrees C and 37 degrees C were used to gain insight into the lateral organization of lipids in bilayers. Analysis of phase diagrams revealed that the phospholipid composition of cholesterol-poor and cholesterol-rich domains reflected the affinity that cholesterol exhibited toward bilayers composed of different lipids. Therefore, the determined affinity of cholesterol for different phospholipid bilayers was useful in predicting the cholesterol-induced lateral segregation of lipids in complex bilayers.
机译：细胞膜中脂质和蛋白质的侧向组织被认为是几种细胞过程中的重要因素。胆固醇被认为是调节脂质横向分离成胆固醇少和胆固醇丰富的区域的功能。我们研究了胆固醇对不同磷脂的亲和力，如在甲基-β-环糊精和大单层囊泡之间的胆固醇分配中所见，如何在复杂双层脂质的横向组织中反映出来。我们特别想确定低T（m）脂质如何影响侧向结构。分区实验表明，胆固醇对N-油酰-鞘磷脂（OSM）的亲和力高于对棕榈酰-油酰-磷脂酰胆碱（POPC）双层的亲和力，但最优先选择的是含N-棕榈酰-鞘磷脂（PSM）的双层。使用POPC / PSM /胆固醇和OSM / PSM /胆固醇双层在23摄氏度和37摄氏度下的部分相图来深入了解双层脂质的横向组织。相图分析表明，胆固醇少和胆固醇丰富的域的磷脂组成反映了胆固醇对由不同脂质组成的双层分子表现出的亲和力。因此，确定的胆固醇对不同磷脂双层的亲和力可用于预测胆固醇诱导的复杂双层中脂质的侧向分离。

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