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首页> 外文期刊>Clinical and experimental medicine >Characterisation of TNF-related apoptosis-inducing ligand in peripheral blood in patients with primary biliary cirrhosis.
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Characterisation of TNF-related apoptosis-inducing ligand in peripheral blood in patients with primary biliary cirrhosis.

机译：原发性胆汁性肝硬化患者外周血中TNF相关凋亡诱导配体的表征。

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Apoptosis plays a pivotal role in portal tract damage of primary biliary cirrhosis (PBC). Tumour necrosis factor-related apoptosis inducing ligand (TRAIL) is an apoptotic inducer, and it has been reported that the expression of TRAIL receptors is up-regulated by increased bile acid level and the serum level of soluble TRAIL (sTRAIL) is elevated in PBC patients. In the present study, we investigated the association of TRAIL in peripheral blood with the pathogenesis of PBC and chronic hepatitis B. The expression levels of TRAIL mRNA and protein on leukocytes and sTRAIL in plasma from 27 patients with PBC, 25 with CHB and 30 healthy controls were determined respectively by real-time fluorescent quantitative reverse transcription polymerase chain reaction (RT-PCR), flow cytometry (FCM) and ELISA. The expression levels of TRAIL mRNA and protein on leukocytes and plasma sTRAIL were all up-regulated in the patients with PBC and CHB compared to controls. In the two diseased groups, TRAIL mRNA showed significant correlation of both membrane-bound TRAIL (mTRAIL) on monocytes and plasma sTRAIL. So did plasma TNF-alpha. In PBC patients, mTRAIL and sTRAIL correlated well with gamma-glutamyltransferase and alkaline phosphatase, but not with aspartate aminotransferase and alanine amino-transferase. The opposite case was found in CHB patients. These results suggested that both mTRAIL and sTRAIL might be involved in the development and progression of PBC and CHB in humans, but the mechanisms might be different.
机译：凋亡在原发性胆汁性肝硬化（PBC）的门道损伤中起关键作用。肿瘤坏死因子相关的凋亡诱导配体（TRAIL）是一种凋亡诱导剂，据报道，胆汁酸水平升高会上调TRAIL受体的表达，而PBC中可溶性TRAIL（sTRAIL）的血清水平会升高耐心。在本研究中，我们调查了外周血中TRAIL与PBC和慢性乙型肝炎的发病机制的关系。27例PBC，25例CHB和30例健康人的血浆中TRAIL mRNA和蛋白在血浆白细胞和sTRAIL中的表达水平分别通过实时荧光定量逆转录聚合酶链反应（RT-PCR），流式细胞仪（FCM）和ELISA确定对照。与对照组相比，PBC和CHB患者的白细胞和血浆sTRAIL上TRAIL mRNA和蛋白的表达水平均上调。在两个患病组中，TRAIL mRNA显示单核细胞上的膜结合TRAIL（mTRAIL）和血浆sTRAIL均显着相关。血浆TNF-α也是如此。在PBC患者中，mTRAIL和sTRAIL与γ-谷氨酰转移酶和碱性磷酸酶相关性很好，而与天冬氨酸氨基转移酶和丙氨酸氨基转移酶无关。在CHB患者中发现相反的情况。这些结果表明，mTRAIL和sTRAIL可能都参与了人类PBC和CHB的发展和进程，但机制可能有所不同。

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