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首页> 外文期刊>Clinical and Experimental Immunology: An Official Journal of the British Society for Immunology >CD4+CD25highforkhead box protein 3+ regulatory T lymphocytes suppress interferon-γ and CD107 expression in CD4+ and CD8+ T cells from tuberculous pleural effusions
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CD4+CD25highforkhead box protein 3+ regulatory T lymphocytes suppress interferon-γ and CD107 expression in CD4+ and CD8+ T cells from tuberculous pleural effusions

机译：CD4 + CD25highforkhead box蛋白3+调节性T淋巴细胞抑制结核性胸腔积液CD4 +和CD8 + T细胞中γ-干扰素和CD107的表达

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Summary: Tuberculous pleural effusion is characterized by a T helper type 1 (Th1) profile, but an excessive Th1 response may also cause tissue damage that might be controlled by regulatory mechanisms. In the current study we investigated the role of regulatory T cells (Treg) in the modulation of Th1 responses in patients with tuberculous (TB) pleurisy. Using flow cytometry we evaluated the proportion of Treg (CD4+CD25highforkhead box protein 3+), interferon (IFN)-γ and interleukin (IL)-10 expression and CD107 degranulation in peripheral blood (PB) and pleural fluid (PF) from patients with TB pleurisy. We demonstrated that the proportion of CD4+CD25+, CD4+CD25highFoxP3+ and CD8+CD25+ cells were increased in PF compared to PB samples. Mycobacterium tuberculosis stimulation increased the proportion of CD4+CD25lowegIL-10+ in PB and CD4+CD25lowegIFN-γ+ in PF; meanwhile, CD25high mainly expressed IL-10 in both compartments. A high proportion of CD4+CD107+ and CD8+CD107+ cells was observed in PF. Treg depletion enhanced the in-vitro M. tuberculosis-induced IFN-γ and CD4+ and CD8+ degranulation responses and decreased CD4+IL-10+ cells in PF. Our results demonstrated that in TB pleurisy Treg cells effectively inhibit not only IFN-γ expression but also the ability of CD4+ and CD8+ cells to degranulate in response to M. tuberculosis.
机译：摘要：结核性胸腔积液的特点是T型辅助1型（Th1），但是过度的Th1反应也可能导致组织损伤，这可能是由调节机制控制的。在当前的研究中，我们调查了结核性胸膜炎患者中调节性T细胞（Treg）在Th1反应调节中的作用。我们使用流式细胞仪评估了患者外周血（PB）和胸膜液（PF）中Treg（CD4 + CD25highforkhead box蛋白3+），干扰素（IFN）-γ和白介素（IL）-10表达以及CD107脱颗粒的比例结核性胸膜炎。我们证明，与PB样品相比，PF中CD4 + CD25 +，CD4 + CD25highFoxP3 +和CD8 + CD25 +细胞的比例增加。结核分枝杆菌刺激增加了PB中CD4 + CD25low / negIL-10 +和PF中CD4 + CD25low egIFN-γ+的比例;同时，CD25high在两个区室中主要表达IL-10。在PF中观察到高比例的CD4 + CD107 +和CD8 + CD107 +细胞。 Treg耗竭增强了体外结核分枝杆菌诱导的IFN-γ和CD4 +和CD8 +脱粒反应，并减少了PF中的CD4 + IL-10 +细胞。我们的结果表明，在结核性胸膜炎中，Treg细胞不仅能有效抑制IFN-γ的表达，而且还能抑制CD4 +和CD8 +细胞对结核分枝杆菌的脱颗粒能力。

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